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  4. Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

  • 中文名稱(chēng)：
    小鼠血管緊張素1-7（Ang1-7）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13763m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血管緊張素1-7（Ang1-7）酶聯(lián)免疫試劑盒（CSB-E13763m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Ang1-7含量。Ang1-7是有研究?jī)r(jià)值的靶點(diǎn)。它在心血管、腎臟等多個(gè)系統(tǒng)發(fā)揮作用，具有舒張血管、抗增殖、抗炎等效應(yīng)。研究機(jī)制方面，主要通過(guò)與Mas受體結(jié)合，激活下游信號(hào)通路，調(diào)節(jié)細(xì)胞功能，改善器官病理生理狀態(tài)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于實(shí)驗(yàn)室對(duì)小鼠疾病模型中Ang1-7的動(dòng)態(tài)監(jiān)測(cè)，如高血壓、心肌肥厚、腎臟損傷等研究的生物標(biāo)志物分析，或體外藥物篩選實(shí)驗(yàn)中細(xì)胞因子分泌水平的定量評(píng)估。Ang1-7水平變化常被用于心血管疾病模型研究、代謝綜合征機(jī)制探索及藥物干預(yù)效果評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Ang1-7
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %93
    Range %87-99
    1:4Average %95
    Range %89-102
    1:8Average %95
    Range %87-98
  • 回收率：
    The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-98
    EDTA plasma (n=4)9591-98
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.745 2.721 2.733 2.597
    2002.316 2.245 2.281 2.145
    1001.923 1.898 1.911 1.775
    501.467 1.366 1.417 1.281
    250.796 0.788 0.792 0.656
    12.50.458 0.447 0.453 0.317
    6.250.258 0.242 0.250 0.114
    00.138 0.134 0.136 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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