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  4. Rat angiotensin 1-7 (Ang1-7) ELISA kit

Rat angiotensin 1-7 (Ang1-7) ELISA kit

  • 中文名稱：
    大鼠血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14241r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒（CSB-E14241r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Ang1-7含量。Ang1-7是一種血管緊張素，作為腎素-血管緊張素系統(tǒng)（RAS）的組成部分，其作用與AngⅡ相反。研究顯示，Ang1-7可通過調(diào)節(jié)Rac1、Rad等蛋白表達(dá)，抑制成纖維細(xì)胞合成細(xì)胞外基質(zhì)，從而對心血管系統(tǒng)產(chǎn)生保護(hù)作用。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于基礎(chǔ)科研中評估心血管疾病模型、代謝紊亂或器官損傷等實驗條件下Ang1 - 7的動態(tài)變化，在高血壓、糖尿病腎病等疾病機(jī)制研究中具有重要價值，滿足實驗室對生物活性分子定量分析的需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Ang1-7
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %80-105
    1:2Average %96
    Range %85-104
    1:4Average %102
    Range %92-114
    1:8Average %99
    Range %80-110
  • 回收率：
    The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9683-112
    EDTA plasma (n=4)9590-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12003.121 3.129 3.125 2.986
    6002.532 2.522 2.527 2.388
    3001.739 1.765 1.752 1.613
    1501.050 1.041 1.046 0.907
    750.623 0.642 0.633 0.494
    37.50.435 0.447 0.441 0.302
    18.750.265 0.254 0.260 0.121
    00.135 0.143 0.139 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

What are host animals of antibodies in the kit? Are the antibodies affinity purified?

A:
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity?purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.