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  4. Mouse Cathepsin S(CTSS) ELISA kit

Mouse Cathepsin S(CTSS) ELISA kit

  • 中文名稱：
    小鼠組織蛋白酶S(CTSS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006204MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠組織蛋白酶S(CTSS)酶聯(lián)免疫試劑盒（CSB-EL006204MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CTSS含量。CTSS（組織蛋白酶S）是一種溶酶體半胱氨酸蛋白酶，參與細胞內(nèi)蛋白質(zhì)降解和抗原呈遞，與免疫系統(tǒng)和多種疾病如神經(jīng)退行性疾病、癌癥和炎癥反應密切相關(guān)。其研究機制主要涉及CTSS在細胞內(nèi)外的表達、活性調(diào)控及其與底物和受體的相互作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本試劑盒為研究CTSS在免疫調(diào)控、炎癥通路或相關(guān)疾病機制中的功能提供高效工具，可應用于基礎科研中的分子機制探索、藥物干預效果評價或生物標志物篩查等方向。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ctss ELISA kit; CatsCathepsin S ELISA kit; EC 3.4.22.27 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-92
    1:2Average %95
    Range %91-105
    1:4Average %96
    Range %90-101
    1:8Average %93
    Range %85-98
  • 回收率：
    The recovery of mouse CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-98
    EDTA plasma (n=4)8783-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.884 2.952 2.918 2.750
    10002.345 2.452 2.399 2.231
    5001.652 1.549 1.601 1.433
    2500.729 0.739 0.734 0.566
    1250.457 0.452 0.455 0.287
    62.50.285 0.297 0.291 0.123
    31.250.216 0.219 0.218 0.050
    00.168 0.167 0.168 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
  • 基因功能參考文獻：
    1. The present data indicating that Cat-S activity increases with CKD progression suggest that Cat-S might be a therapeutic target to prevent cardiovascular complications in CKD. PMID: 28240259
    2. CatS(-/-) mice exhibited impaired social interaction and social novelty recognition in the three-chamber test. PMID: 28623134
    3. Cathepsin S activity controls injury-related vascular remodeling via TLR2/p38MAPK/PI3K/Akt/p-HDAC6 signaling pathway. PMID: 27365406
    4. Ctss induction during muscular dystrophy is a pathologic event that partially underlies disease pathogenesis, and its inhibition might serve as a new therapeutic strategy in Duchenne muscular dystrophy. PMID: 26966179
    5. Fluorogen substrate, Mca-GRWPPMGLPWE-Lys(Dnp)-DArg-NH2 can detect CTSS activities in mouse antigen presenting cells. PMID: 26899824
    6. Data show that cathepsins S (CatS) regulates CCL2 chemokine expression by modulation of CD74 antigen processing. PMID: 26358505
    7. Cathepsin S activates MrgprC11 and evokes receptor-dependent scratching in mice. PMID: 26216096
    8. cathepsin S deficiency alters the balance between adipocyte and osteoblast differentiation, increases bone turnover, and changes bone microarchitecture. Therefore, bone and fat metabolisms should be monitored when using cathepsin S inhibitors clinically PMID: 24780878
    9. results identify Cat-S as a biased agonist of PAR2 that causes PAR2- and TRPV4-dependent inflammation and pain. PMID: 25118282
    10. Cathepsin S contributes to macrophage migration via degradation of elastic fibre integrity to facilitate neointima formation of vein grafts PMID: 24319016
    11. CatS also reduced myocardial Smad2 and Smad3 activation and extra domain A fibronectin expression PMID: 23771947
    12. CatS is involved in the secondary injury after traumatic brain injury PMID: 24282339
    13. Disruption of CatS therefore induces hyperlocomotor activity due to failure to downscale the synaptic strength. PMID: 24067868
    14. These results show a peripheral pivotal role of CatS in the development of neuropathic pain through the antigen-specific activation of CD4(+) T-cells PMID: 24553941
    15. High cathepsin S promotes cancer growth and neovascularization. PMID: 23629809
    16. Provide direct evidence that Cat S plays an important role in abdominal aortic aneurysm formation in apoE-deficient mice. PMID: 22871592
    17. GILT expression decreased the proteolysis of a CatS selective substrate. PMID: 23012103
    18. Cathepsin S deficiency results in abnormal accumulation of autophagosomes in macrophages and enhances Ang II-induced cardiac inflammation. PMID: 22558139
    19. Overexpression of cathepsin S induces chronic atopic dermatitis in mice. PMID: 22170489
    20. Cat-S activates nociceptors to induce visceral pain via protease-activated receptor-2. PMID: 21802389
    21. only prophylactic Cat S inhibitor dosing blocked lung inflammation, consistent with our findings in Cat S knockout mice. PMID: 20855652
    22. Cathepsin S inhibition prevented the formation of lung granulomas in a mouse model of sarcoidosis. PMID: 21251246
    23. Cat S derived from macrophages is involved in the mechanisms of atherosclerotic plaque vulnerability in apoE-deficient mice PMID: 20079903
    24. For a subset of antigens, epitope generation is critically regulated by cathepsin S, which participates in antigen processing and generates qualitative and quantitative differences in the peptide repertoires displayed by MHC class II molecules. PMID: 11884425
    25. regulation of the phagosomal CatS, CatL, CatB and CatZ contents during dendritic cell activation PMID: 12186844
    26. cathepsin S contributes to angiogenesis by microvascular endothelial cells. PMID: 12600886
    27. In vivo, nonprofessional histocompatibility class (MHC) II-expressing antigen presenting cells of the intestinal epithelium use Cat S, but not Cat L, for MHC class II-mediated antigen presentation. PMID: 15661874
    28. First direct genetic evidence is reported for a key role of cathepsin S in autoimmune responses to acetylcholine receptor and in the pathogenesis of experimental autoimmune myasthenia gravis. PMID: 15661938
    29. Transgenic interferon-gamma induces alveolar epithelial cell DNA injury and apoptosis via a novel murine cathepsin S-dependent pathway, a critical event in the pathogenesis of alveolar remodeling, emphysema and inflammation. PMID: 15944319
    30. The increases in mRNA levels for CatS and GPX3 found in the aging C57BL/6 RPE/choroid appear to represent an increase in both the numbers of cells expressing these messages and an increase in the level of expression in individual cells. PMID: 15947738
    31. Cathepsin S has a unique role in determining the morphology of class II MHC-positive endosomal compartments PMID: 16094690
    32. interleukin-6-signal transducer and activator of transcription 3 -mediated increase of cathepsin S activity PMID: 16286017
    33. Data show that cathepsin S deficiency impairs angiogenesis and tumor cell proliferation, impairing angiogenic islet formation and the growth of solid tumors; while the absence of its endogenous inhibitor cystatin C resulted in opposite phenotypes. PMID: 16365041
    34. plays an important role in atherosclerotic plaque destabilization and rupture PMID: 16410454
    35. Plays role in migration and activation of microglia to protect facial motoneurons against axotomy-induced injury. PMID: 17539023
    36. These results indicate that seizures induced by kainate elicit neurodegeneration, astrogliosis, and microglial activation accompanied by the expression of cathepsin S while those induced by nicotine do not. PMID: 17997037
    37. Upregulation of elastase proteins results in aortic dilatation in mucopolysaccharidosis I mice. PMID: 18479957
    38. CAT S participates in inflammatory processes accompanying aging and pathologies of the central nervous system PMID: 18694734
    39. Leukocyte cathepsin S is a potent regulator of both cell and matrix turnover in advanced atherosclerosis. PMID: 19095996
    40. catS-induced elastolysis accelerates arterial and aortic valve calcification in chronic renal disease PMID: 19307473
    41. The active form of cathepsin S was identified as being significantly up-regulated in poorly differentiated TRAMP tumours. PMID: 19487544

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  • 亞細胞定位：
    Lysosome. Secreted. Cytoplasmic vesicle, phagosome.
  • 蛋白家族：
    Peptidase C1 family
  • 組織特異性：
    Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues. Expressed in spleen, B cells, dendritic cells and macrophages.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:13040

    STRING: 10090.ENSMUSP00000112006

    UniGene: Mm.3619