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  4. Mouse Ferritin heavy chain(FTH1) ELISA kit

Mouse Ferritin heavy chain(FTH1) ELISA kit

  • 中文名稱：
    小鼠鐵蛋白重鏈(FTH1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL009030MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠鐵蛋白重鏈(FTH1)酶聯(lián)免疫試劑盒（CSB-EL009030MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FTH1含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于科研領(lǐng)域中對(duì)鐵代謝機(jī)制、氧化損傷模型、肝臟功能研究以及鐵相關(guān)基因調(diào)控實(shí)驗(yàn)的樣本分析，為探索FTH1在生理病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Fth1 ELISA Kit; FthFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) [Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FTH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 88-99  
    1:2 Average % 97  
    Range % 92-103  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 89  
    Range % 83-95  
  • 回收率：
    The recovery of mouse FTH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 98 95-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.178 2.162 2.170 2.012  
    10 1.713 1.695 1.704 1.546  
    5 1.085 1.128 1.107 0.949  
    2.5 0.693 0.751 0.722 0.564  
    1.25 0.397 0.388 0.393 0.235  
    0.625 0.254 0.267 0.261 0.103  
    0.312 0.193 0.201 0.197 0.039  
    0 0.154 0.161 0.158 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 基因功能參考文獻(xiàn)：
    1. FtMt overexpressing mice have no significant defects and the overexpression of FtMt does not affect the regulation of iron metabolism significantly in transgenic mice. PMID: 28703745
    2. Ferritin is considered the major iron storage protein which maintains a large iron core in its cavity and has ferroxidase activity. (Review) PMID: 28349628
    3. the absence of Mitochondrial ferritin, which is highly expressed in the heart, increases the sensitivity of mitochondria to cardiac injury via oxidative stress. PMID: 27853170
    4. As previously reported, homozygous loss of the Fth allele on a wild-type Ftl background was embryonic lethal. expression of the H subunit can rescue the loss of the L subunit and that H ferritin homopolymers have the capacity to sequester iron in vivo PMID: 25629408
    5. provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
    6. Our data show that ferritin H is required for B and T cell survival by actively reducing the labile iron pool. PMID: 24586648
    7. Ferritin H deficiency in most cells of the forebrain including cells of the choroid plexus caused accumulation of cerebrospinal fluid in the lateral ventricles and the subarachnoid space PMID: 24662374
    8. Mycobacterium avium infection induces H-ferritin expression in mouse primary macrophages by activating Toll-like receptor 2. PMID: 24349383
    9. 3-Hydroxybutyrate dehydrogenase-2 and ferritin-H synergistically regulate intracellular iron. PMID: 24673886
    10. Fth plays a critical protective role during acute kidney injury. PMID: 24018561
    11. FtH provides metabolic adaptation to tissue Fe overload, conferring tolerance to malaria PMID: 23159058
    12. The delivery of iron to oligodendrocytes via H-ferritin is a biologically relevant delivery system, given that ferritin--not transferrin--receptors can be demonstrated on oligodendrocytes in vivo. PMID: 21446040
    13. HFt taken up by TIM-2 positive cells transited through the endosome and eventually entered a lysosomal compartment, distinguishing the HFt pathway from that of transferrin PMID: 21886823
    14. Mitochondrial ferritin is found in all regions of the brain in C57/BL6 mice; the role of mitochondrial ferritin can be expected to contribute to our knowledge of mitochondrial dysfunction and neurodegenerative disease. PMID: 20722075
    15. Loss of one allele of H ferritin appears to increase light-induced degeneration. PMID: 20881284
    16. Hx plays an important role in controlling iron distribution within brain, thus suggesting its involvement in iron-related neurodegenerative diseases. PMID: 19120692
    17. FTH expression is mediated by Nrf2 in response to xenobiotics and cancer chemopreventive dithiolethiones PMID: 12435735
    18. Ferritin heavy chain 1 necessary for tumor necrosis factor alpha-induced apoptosis in L929 cells. PMID: 16024802
    19. excessive free iron and the resultant enhanced oxidative stress caused by downregulation of FHC lead to cardiomyocyte death PMID: 18992754

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  • 蛋白家族：
    Ferritin family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:14319

    STRING: 10090.ENSMUSP00000025563

    UniGene: Mm.1776