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  4. Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

  • 中文名稱：
    小鼠半乳糖凝集素3結(jié)合蛋白(LGALS3BP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL012888MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠半乳糖凝集素3結(jié)合蛋白(LGALS3BP)酶聯(lián)免疫試劑盒（CSB-EL012888MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LGALS3BP含量。LGALS3BP是重要的靶點。它在多種疾病尤其腫瘤進程里扮演關(guān)鍵角色，和腫瘤細胞增殖、轉(zhuǎn)移及免疫逃逸相關(guān)。研究機制方面，主要聚焦其分子結(jié)構(gòu)與功能，以及和其他生物分子的相互作用，旨在開發(fā)針對性治療藥物。試劑盒檢測范圍為0.39 ng/mL-25 ng/mL，適用于基礎(chǔ)研究領(lǐng)域，如探索LGALS3BP在免疫相關(guān)疾病模型中的表達變化、評估其在炎癥信號通路中的作用機制，或作為腫瘤微環(huán)境研究的潛在分子標記物檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Lgals3bp ELISA Kit; Cycap ELISA Kit; MamaGalectin-3-binding protein ELISA Kit; Cyp-C-associated protein ELISA Kit; CyCAP ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; Protein MAMA ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.39 ng/mL-25 ng/mL
  • 靈敏度：
    0.09 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 94
    Range % 90-98
    1:2000 Average % 102
    Range % 96-107
    1:4000 Average % 87
    Range % 81-92
    1:8000 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of mouse LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 97-107
    EDTA plasma (n=4) 98 92-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    25 2.867 2.723 2.795 2.707
    12.5 1.988 1.856 1.922 1.834
    6.25 1.258 1.219 1.239 1.151
    3.12 0.734 0.715 0.725 0.637
    1.56 0.468 0.479 0.474 0.386
    0.78 0.242 0.248 0.245 0.157
    0.39 0.152 0.159 0.156 0.068
    0 0.088 0.087 0.088 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • 基因功能參考文獻：
    1. These findings suggest a novel immunoinhibitory function for LGALS3BP that might be important for immune evasion of tumor cells during cancer progression. PMID: 25320078
    2. Data give novel evidence that CyCAP regulates the post-translational modification of tissue transglutaminase through its colocalization with calnexin in endoplasmic reticulum. PMID: 20049854
    3. Cyclophilin C-associated protein is expressed in skin and during wound repair. It is regulated by IFNgamma and may function on immune defense in macrophages, lymphocytes, dermal fibroblasts and keratinocytes during wound healing. PMID: 16998803
    4. CyCAP-deficient mice spontaneously and after challenge with carcinogen develop significantly more colorectal mucosal hyperplasia, an early stage in murine colonic carcinogenesis PMID: 19627619
  • 亞細胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Detected in embryo, liver, spleen, kidney, lung, heart, intestine, thymus and lymph node.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:19039

    STRING: 10090.ENSMUSP00000035579

    UniGene: Mm.3152