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  4. Mouse Hyaluronic acid,HA ELISA Kit

Mouse Hyaluronic acid,HA ELISA Kit

  • 中文名稱(chēng)：
    小鼠透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08121m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒（CSB-E08121m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HA含量。HA（血凝素）是流感病毒表面的糖蛋白，在病毒感染宿主細(xì)胞過(guò)程中起關(guān)鍵作用。其可與宿主細(xì)胞表面受體結(jié)合，介導(dǎo)病毒膜與細(xì)胞膜融合。研究HA有助于揭示流感病毒感染機(jī)制，為流感疫苗研發(fā)、抗病毒藥物設(shè)計(jì)提供重要靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于科研領(lǐng)域?qū)A表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)。在關(guān)節(jié)炎模型、皮膚再生研究、腫瘤微環(huán)境分析及代謝相關(guān)疾病機(jī)制探索中具有重要研究?jī)r(jià)值。適用于比較不同病理狀態(tài)下HA分泌水平差異或評(píng)估干預(yù)措施對(duì)HA代謝的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    HA
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %82-99
    1:200Average %93
    Range %89-101
    1:400Average %102
    Range %98-106
    1:800Average %97
    Range %92-102
  • 回收率：
    The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)9793-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.755 2.856 2.806 2.652
    20002.027 2.128 2.078 1.924
    10001.435 1.455 1.445 1.291
    5000.850 0.861 0.856 0.702
    2500.526 0.515 0.521 0.367
    1250.358 0.348 0.353 0.199
    62.50.267 0.261 0.264 0.110
    00.154 0.153 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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