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  4. Rat Hyaluronic acid,HA ELISA Kit

Rat Hyaluronic acid,HA ELISA Kit

  • 中文名稱：
    大鼠透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08120r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒（CSB-E08120r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HA含量。靶點(diǎn)研究是醫(yī)藥創(chuàng)新的關(guān)鍵環(huán)節(jié)，通過(guò)深入疾病分子機(jī)制和生物學(xué)特性，尋找新的藥物靶點(diǎn)，設(shè)計(jì)精確治療方案。研究機(jī)制包括基因組學(xué)、蛋白質(zhì)組學(xué)和生物信息學(xué)，以及高通量篩選技術(shù)，旨在發(fā)現(xiàn)和開(kāi)發(fā)高效、選擇性的藥物。試劑盒檢測(cè)范圍為4.69 ng/mL-300 ng/mL，適用于探索HA在組織再生、慢性炎癥或代謝性疾病中的動(dòng)態(tài)變化，如通過(guò)檢測(cè)肝病模型中的血清HA水平分析纖維化進(jìn)展，或評(píng)估關(guān)節(jié)炎動(dòng)物模型中滑膜組織勻漿的HA代謝異常。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    N/A
  • 縮寫：
    HA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    4.69 ng/mL-300 ng/mL
  • 靈敏度：
    1.17 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %90-96
    1:2Average %91
    Range %87-96
    1:4Average %97
    Range %93-100
    1:8Average %89
    Range %85-96
  • 回收率：
    The recovery of rat HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9390-96
    EDTA plasma (n=4)9590-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    3002.656 2.678 2.667 2.505
    1501.848 1.892 1.870 1.708
    751.202 1.264 1.233 1.071
    37.50.763 0.778 0.771 0.609
    18.750.496 0.473 0.485 0.323
    9.380.384 0.371 0.378 0.216
    4.690.275 0.298 0.287 0.125
    00.161 0.163 0.162 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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