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  4. Mouse Interleukin 1α,IL-1α ELISA kit

Mouse Interleukin 1α,IL-1α ELISA kit

  • 中文名稱：
    小鼠白介素1α(IL-1α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04621m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠白介素1α(IL-1α)酶聯(lián)免疫試劑盒（CSB-E04621m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL1A含量。IL1A，即白介素-1α，是一種重要的細(xì)胞因子，參與調(diào)節(jié)免疫和炎癥反應(yīng)。其研究機(jī)制涉及前體蛋白的轉(zhuǎn)錄因子功能及成熟形式的信號(hào)傳導(dǎo)，對(duì)細(xì)胞生長(zhǎng)、分化和免疫應(yīng)答有顯著影響。試劑盒檢測(cè)范圍為2.35 pg/ml- 150 pg/ml，靈敏度為0.58 pg/ml。本試劑盒適用于科研領(lǐng)域中小鼠模型的炎癥機(jī)制研究、免疫相關(guān)疾病動(dòng)物模型的病理評(píng)估、藥物干預(yù)效果分析等實(shí)驗(yàn)場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Il1aInterleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    2.35 pg/ml - 150 pg/ml
  • 靈敏度：
    0.58 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    18.596

    18.529

    18.512

    18.426

    18.546

    18.495

    SD

    0.041

    0.046

    0.044

    0.048

    0.056

    0.054

    CV(%)

    3.010

    3.387

    3.242

    3.551

    4.121

    3.982

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    93-105

    1:2

    Average %

    92

    Range %

    87-99

    1:4

    Average %

    95

    Range %

    90-99

    1:8

    Average %

    93

    Range %

    88-96

  • 回收率：

    The recovery of mouse IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    90-98

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    150

    2.901

    2.887

    2.894

    2.72

    75

    2.461

    2.345

    2.403

    2.229

    37.5

    1.971

    1.828

    1.900

    1.755

    18.75

    1.362

    1.278

    1.32

    1.146

    9.38

    0.717

    0.689

    0.703

    0.529

    4.69

    0.458

    0.447

    0.453

    0.279

    2.35

    0.298

    0.281

    0.29

    0.116

    0

    0.175

    0.173

    0.174

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    IL1A是一種細(xì)胞因子，具有調(diào)節(jié)炎癥和免疫反應(yīng)的作用。最新研究發(fā)現(xiàn)，IL1A不僅在炎癥和免疫相關(guān)疾病中發(fā)揮重要作用，還可能參與癌癥的發(fā)生和進(jìn)展。
  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能參考文獻(xiàn)：
    1. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
    2. Together, these data suggest that caspase-11/IL-1alpha pathway plays an important role in defending against Klebsiella pneumoniae by recruiting neutrophils in the early stage of infection. PMID: 28939441
    3. These data highlight an important interdependency between the potent pro-inflammatory cytokine IL1A and Fshr expression. PMID: 28337831
    4. Since neither IL-1alpha nor IL-1beta depletions completely rescued the phenotype, we believe that IL-1alpha and IL-1beta have a similar and probably complementary role in FHF progression PMID: 28953903
    5. These results suggested that Streptococcus pneuomoniae PLY induces the influx of calcium in Streptococcus pneumoniae-infected macrophages, followed by calpain activation and subsequent IL-1alpha maturation and secretion. PMID: 28630064
    6. In response to chemically induced colitis, this microbial landscape promoted the release of IL-1alpha, which acted as a critical driver of colitis and colitis-associated cancer. PMID: 27775548
    7. our results suggest that mature IL-1alpha induced by hS100A7 is via RAGE-p38 MAPK and calpain-1 pathway in keratinocyte and this mechanism may play an important role during psoriasis. PMID: 28060905
    8. Il-1 signaling pathway has a key role in abdominal aortic aneurysm formation in mouse model of Kawasaki disease. PMID: 26941015
    9. endothelial cells were identified as the primary cellular source of G-CSF during OPC, which responded to IL-1alpha that was released from keratinocytes in the infected tissue. PMID: 27632536
    10. Key aspects of IL-1alpha biology and regulation especially with regard to inflammation are reviewed. Review. PMID: 27434011
    11. data suggested that pINSd needs IL-1R1 for inflammatory cytokine induction. Mouse embryo fibroblast cells of IL-1R1-deficient mice further confirmed that pINSd promotes immune responses through IL-1R1 PMID: 27226621
    12. IL-1alpha signaling and DNA damage is important for triggering a sterile inflammatory cascade . PMID: 26439902
    13. As a dual function cytokine, IL-1alpha may contribute to the induction of CHOP intracellularly, while IL-1alpha released from necrotic cells accelerates steatohepatitis via induction of inflammatory cytokines by neighboring cells. PMID: 26022690
    14. These data demonstrate that DC and macrophages display distinct patterns of cytokine regulation, particularly with respect to IL-1, as a consequence of cell-type specific differences in the physicochemical properties of the P2X(7)R PMID: 26068648
    15. Data suggest the role of stromal cell IL-1alpha and IL-1beta in Kawasaki disease vasculitis model. PMID: 26515418
    16. IL-1alpha and IL-36alpha form a self-amplifying inflammatory loop in vivo that in patients with insufficient counter regulatory mechanisms may become hyper-engaged and/or chronic PMID: 26203636
    17. IL-1alpha-positive cells were identified in the epithelium in dextran sulfate sodium (DSS)-induced colitis. IL-1alpha was detected in the stool of colitic mice before IL-1beta. PMID: 25864926
    18. IL-1alpha acts as an alarmin essential for leukocyte recruitment and protective immunity against HSV-1 PMID: 25323745
    19. The aim of this study was to characterize the role of IL-1 in cellular responses of carbon nanotubes in cells from IL-1alpha/beta wild type (IL1-WT) mice. PMID: 25748835
    20. These findings do not support the previously suggested role of nuclear IL-1alpha in gene regulation of IL-1beta. PMID: 25748836
    21. the underlying mechanism by which AR influences AAA development is through IL-1alpha and transforming growth factor-beta1, and provides a potential new therapy to suppress/prevent AAA by targeting AR with ASC-J9. PMID: 26324502
    22. inhibition of this potentially important source of chronic inflammation in atherosclerosis requires blockade of interleukin-1alpha and not interleukin-1beta. PMID: 26139463
    23. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PMID: 25058342
    24. data suggest that central inhibition of IL-1alpha or Tox3 overexpression during the acute phase of a CNS insult may be an effective means for preventing the loss of neurological function PMID: 26224856
    25. Data (including date from studies in knockout mice) suggest that neutralization/deletion of Il1a reduces Il1b production and neutrophil infiltration in lung after inhalation exposure to silica nanoparticles. PMID: 25497724
    26. The controlled release of IL-1alpha could be a critical regulator during acute liver inflammation PMID: 25870999
    27. it appears the balance between TPO and IL-1alpha determines the MK cellular programming for thrombopoiesis in response to acute and chronic platelet needs. PMID: 25963822
    28. In acute lung injury, lipopolysaccharide induced alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1alpha release. PMID: 25862090
    29. IL-1alpha induced the proliferation of CD11b(low) alveolar macrophages and differentiated these cells into CD11b(high) macrophages which perform critical phagocytic functions and organize granuloma. PMID: 25421226
    30. Data indicate that interleukin-1 cytokines IL-1alpha and IL-1beta are regulated by polyubiquitination and proteasomal degradation. PMID: 25371210
    31. IL-1 is a key mediator driving an innate immune response to inflammatory challenge in the mouse brain but is dispensable in extracerebral tissues including the lung and peritoneum. PMID: 25367678
    32. findings identify IL-1alpha as a crucial early danger signal triggering post-MI inflammation. PMID: 25505286
    33. Immune complexes inhibit IL-1 secretion and inflammasome activation. PMID: 25320279
    34. Selective deficiency of IL-1alpha in Kupffer cells reduces liver inflammation and expression of inflammatory cytokines, which may implicate Kupffer cell-derived IL-1alpha in steatohepatitis development. PMID: 24582082
    35. cigarette smoke-induced neutrophilia was dependent on IL-1alpha produced by alveolar macrophages and alveolar macrophages isolated from smoke-exposed mice were primed for excessive IL-1alpha production in response to bacterial ligands. PMID: 25092891
    36. tested the possible role of the proinflammatory cytokine IL-1 in the age-related exhaustion of ovarian reserve using IL-1alpha and IL-1beta-KO mice PMID: 25114230
    37. This study documents distinct roles for IL-1alpha and IL-1beta in the response to Pseudomonas aeruginosa infection as a function of the type 3 secretion system effectors produced by the infecting strain. PMID: 25069982
    38. necroptosis caused the processing and release of IL-1alpha, and this was independent of IL-1beta processing and release PMID: 24790078
    39. IL-1 induces systemic inflammation and augments Streptococcal pneumoniae infection, atherosclerosis, and ischemic brain injury via platelet activation and microvascular coagulation. PMID: 24644058
    40. IL-1alpha was not released upon inflammasome activation unless significant cell damage occurred. PMID: 23684408
    41. haematopoietic-derived IL-1 is a key driver of ischaemic brain injury. PMID: 23519030
    42. In severe S. aureus bacteraemia in mice, TNF-alpha, IL-1alpha, and KC are biomarkers predicting fatal outcome of infection. PMID: 23520553
    43. Our results establish IL-1alpha as a critical initiator of the inflammatory response to L. pneumophila PMID: 23686480
    44. discovery of a novel inflammatory circuit in which RIP1-mediated IL-1alpha secretion in response to deregulated SHP-1 activity triggers an inflammatory destructive disease that proceeds independently of inflammasomes and programmed necrosis PMID: 23708968
    45. Results suggest importance of IL-1R1/IL-1alpha to the recruitment and activation of dendritic cells in response to cigarette smoke exposure. PMID: 22992200
    46. ERalpha-regulated repression of TNFalpha and IL1alpha is important for lumen formation and maintenance. PMID: 22328525
    47. Allergic sensitization to HDM was abolished in vivo when IL-1alpha, GM-CSF, or IL-33 was neutralized. PMID: 22802353
    48. Autophagy has a potentially pivotal role to play in the induction and regulation of inflammatory responses by innate immune cells, largely driven by IL-1 and its consequential effects on IL-23 secretion. PMID: 22972933
    49. Vascular wall resident cells are the main targets for the pro-atherogenic effects of bone marrow-derived IL-1 through IL-1R1, partly by induction of adhesion and chemotactic molecules in endothelial cells. PMID: 22236482
    50. This study demonstrated here in in vivo experiments that IL-1 exacerbates the effects of SCI by accentuating the impact of the inflammatory responses. PMID: 22483094

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  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接：