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Mouse Interleukin-7,IL-7 ELISA kit

  • 中文名稱:
    小鼠白介素7(IL-7)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E10257m-IS
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠白介素7(IL-7)酶聯(lián)免疫試劑盒(CSB-E10257m-IS)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL7含量。IL7是重要的靶點。它是一種細胞因子,在淋巴細胞發(fā)育、生存及穩(wěn)態(tài)維持中起關(guān)鍵作用。相關(guān)研究機制聚焦于其信號通路,旨在通過干預(yù)該靶點調(diào)節(jié)免疫系統(tǒng)功能,有望為免疫相關(guān)疾病如癌癥、自身免疫病等治療帶來新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,靈敏度為7.81 pg/mL。廣泛應(yīng)用于免疫學(xué)基礎(chǔ)研究,例如探究感染性疾病模型中IL-7的動態(tài)變化、評估藥物干預(yù)對免疫細胞功能的影響,或分析腫瘤微環(huán)境中細胞因子的調(diào)控機制。其兼容多種生物樣本的特性,尤其適合用于小鼠模型的組織微環(huán)境研究與體液標(biāo)志物檢測,為免疫相關(guān)疾病的分子機制探索提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Il7 ELISA Kit; Il-7 ELISA Kit; Interleukin-7 ELISA Kit; IL-7 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    98

    Range %

    91-105

    1:2

    Average %

    95

    Range %

    88-102

    1:4

    Average %

    92

    Range %

    85-99

    1:8

    Average %

    90

    Range %

    85-96

  • 回收率:

    The recovery of mouse IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    88-101

    EDTA plasma (n=4)

    88

    81-95

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.207

    2.334

    2.271

    2.079

    1000

    1.571

    1.667

    1.619

    1.427

    500

    1.154

    1.088

    1.121

    0.929

    250

    0.649

    0.675

    0.662

    0.470

    125

    0.486

    0.501

    0.493

    0.302

    62.5

    0.385

    0.373

    0.379

    0.187

    31.25

    0.255

    0.254

    0.255

    0.063

    0

    0.194

    0.190

    0.192

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • 基因功能參考文獻:
    1. We engineered CAR-T cells to express interleukin (IL)-7 and CCL19 (7 x 19 CAR-T cells), as these factors are essential for the maintenance of T-cell zones in lymphoid organs... Following treatment of mice with 7 x 19 CAR-T cells, both recipient conventional T cells and administered CAR-T cells generated memory responses against tumors. PMID: 29505028
    2. A key role of the IL7 and Interferon type I receptor axis in the regulation of intratumoral t-cell functions and in the development of primary breast tumor growth and metastasis. PMID: 29070614
    3. BMP4 and IL7 appear to be involved in the interaction between intestinal epithelial cells and intestinal epithelial cells and in the mechanism underlying intestinal mucosal barrier dysfunction. PMID: 29436597
    4. This finding warrants future development of IL-21 and IL-7 co-expressing whole-cell cancer vaccines and their relevant combinatorial regimens. PMID: 27571893
    5. both Flt3 ligand (FL) and IL-7 regulate B-cell commitment in a permissive manner: FL by inducing proliferation of Ly6D(+)CD135(+)CD127(+)CD19(-) progenitors and IL-7 by providing survival signals to these progenitors PMID: 27911806
    6. the in vivo biological role of m(6)A modification in T-cell-mediated pathogenesis and reveals a novel mechanism of T cell homeostasis and Il-7 signal-dependent induction of mRNA degradation PMID: 28792938
    7. IL-7/IL-7R signaling pathway plays a possible role in recurrent pregnancy loss by upregulating Th17 immunity while downregulating Treg immunity. PMID: 27767237
    8. this study shows that IL-7 homeostasis is achieved through consumption by multiple subsets of innate and adaptive immune cells PMID: 28723549
    9. lymphatic vessel expansion occurs in two distinct phases; the first wave of expansion is dependent on IL-7; the second phase, responsible for leukocyte exit from the glands, is regulated by lymphotoxin (LT)betaR signaling PMID: 27474071
    10. DNM2 mutations cooperate with Lmo2 T-cell oncogenes by enhancing IL-7 signalling. PMID: 27118408
    11. IL-7 responsiveness in RTE is designed to maximize survival at the expense of reduced proliferation, consistent with RTE serving as a subpopulation of T cells rich in diversity but not in frequency. PMID: 27129922
    12. Expression of IL-7 is beneficial for induction of potent and long-lasting humoral immune responses. PMID: 28100620
    13. findings support a redundant role for adaptive Th17 cell- and innate gammadeltaT17 cell-derived IL17 in bacteria-induced colon carcinogenesis, stressing the importance of therapeutically targeting the cytokine itself rather than its cellular sources PMID: 26880802
    14. functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells. PMID: 27023180
    15. continuous IL7 signaling was not required for tumor regression, although LIP of naive CD8+ T cells is usually regulated by IL7 PMID: 26880265
    16. IL7 represses the follicular helper T cell gene program. PMID: 26743592
    17. This study uncovers the metabolic mechanisms by which IL-7 tailors the metabolism of memory T cells to promote their longevity and fast response to rechallenge. PMID: 25957683
    18. IL-7 reduced IRAK-M expression and attenuated immune tolerance induced by either LPS or CpGA PMID: 26218271
    19. Poly I:C induces IL-7 production, early inflammatory responses, and characteristic pathologies of SS-like dacryoadenitis in non-autoimmune-prone C57BL/6 mice. PMID: 26658504
    20. Data show that interleukin 7 (IL-7) signaling is a prerequisite for optimal CD4(+) T cell activation. PMID: 26319414
    21. reduced surface expression of IL-7R and concomitant limited responsiveness to IL-7 signals as a common mechanism resulting in reduced cell survival of common lymphoid progenitors and thymocytes at the double-negative to double-positive transition PMID: 26475928
    22. Hair follicle expression of IL-7 was required for CD8+ and CD4+ skin-resident memory T (TRM) cells to exert tropism for the epidermis. In a cutaneous T cell lymphoma model, CD4+ TRM lymphoma cell localization depended on hair follicle-derived IL-7. PMID: 26479922
    23. Data show the contribution of IL-23/IL-23 receptor and IL-7/IL-7 receptor signaling in Th17 and Th1 cell dynamics during experimental autoimmune encephalomyelitis (EAE). PMID: 26223651
    24. IL-7 plays a major role in innate immunity against Citrobacter rodentium infection. PMID: 26034215
    25. The effects of 6-formylindolo (3,2-b) carbazole (Ficz), a ligand of aryl hydrocarbon receptor, on IL-7 expression, colitis and lymphocyte phenotypes are reported. PMID: 25799939
    26. IL-7 critically acts cooperatively with signaling via the pre-TCR and Notch1 to coordinate proliferation, differentiation and TCRalpha recombination during beta-selection. PMID: 25729925
    27. The enhanced thymic reconstitution in the rIL-7/HGFbeta-treated allogeneic BMT recipients results in increased number and functional activities of peripheral T cells. PMID: 24349415
    28. these results suggest that PU.1 and Spi-B activate Btk to oppose IL-7 responsiveness in developing B cells. PMID: 25505273
    29. Results provided evidence that IL-7/IL-7R induce VEGF-D upregulation and promote lymphangiogenesis via c-Fos/c-Jun pathway in lung cancer. PMID: 24115038
    30. OX40 and IL-7 play synergistic, but distinct roles in the homeostatic proliferation of CD4(+) effector memory T cells PMID: 25103720
    31. IL-7 holds promise as a critical cytokine for selectively inducing Tfh cell generation. PMID: 24899182
    32. NF-kappaB has a role in controlling IL-7 responsiveness of quiescent naive T cells PMID: 24799710
    33. IL-12 induces the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
    34. Ikaros is a central regulator of IL-7 signaling and pre-B cell development PMID: 24297995
    35. our data point toward an unexpected new role for IL-7 as a potential autocrine mediator of lymphatic drainage PMID: 23963040
    36. KGF could up-regulate IL-7 expression through the STAT1/IRF-1, IRF-2 signaling pathway, which is a new insight in potential effects of KGF on the intestinal mucosal immune system. PMID: 23554911
    37. -7 enhances the Th1 response to promote the development of Sjogren's syndrome-like autoimmune exocrinopathy in mice. PMID: 23666710
    38. IRFs activated by lymphocyte adhesion induce IL-7 transcription through ISRE in stromal cells. PMID: 23376291
    39. our results suggest that thymic epithelial cell-derived IL-7 plays a major role in proliferation, survival, and maturation of thymocytes and is indispensable for gammadelta T cell development PMID: 23686483
    40. We have analyzed the discrete contributions of the antibody constant (Fc) and IL-7-binding (Fab) domains to the mechanism. PMID: 23610371
    41. expression of IL-7/IL-7R is strongly correlated with rheumatoid arthritis activity and ligation of IL-7 to IL-7R contributes to monocyte homing, differentiation of osteoclasts, and vascularization in the collagen-induced arthritis effector phase. PMID: 23606539
    42. IL-7 could be an important mediator in arthritic conditions PMID: 22676399
    43. Cessation of the IL-7 response of pre-B cell signaling components is controlled via a cell-autonomous mechanism that operates at a discrete developmental transition marked by transient expression of c-Myc. PMID: 23420891
    44. Interleukin-7, but not thymic stromal lymphopoietin, plays a key role in the T cell response to influenza A virus. PMID: 23189186
    45. Interleukin-7 supports survival of T-cell receptor-beta-expressing CD4(-) CD8(-) double-negative thymocytes. PMID: 23215679
    46. poly I:C boosts the T cell immune response in the lung by inducing local IL-7 production, which in turn, enhances T cell-derived IFN-gamma to promote macrophage recruitment, CXCR3 ligand expression, and T cell infiltration. PMID: 23271706
    47. Data show that lymphatic endothelial cells (LECs) are a prominent source of IL-7 both in human and murine lymph nodes. PMID: 22955921
    48. This is the first demonstration that high levels of IL-7 antagonize Notch-1 signaling and suggest that IL-7 may affect T- versus B-lineage choice in the thymus. PMID: 22899673
    49. Although IL-7 is crucial for naive CD4+ T cell homeostatic proliferation in response to lymphopenia, it has minimal impact on the homeostatic proliferation of regulatory CD4+ (Treg) cells. PMID: 22933631
    50. These observations establish a key role for IL-7 in the complex mechanisms by which immune mediators modulate metabolic functions. PMID: 22768283

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    IL-7/IL-9 family
  • 數(shù)據(jù)庫鏈接:

    KEGG: mmu:16196

    STRING: 10090.ENSMUSP00000126219

    UniGene: Mm.3825