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  4. Mouse Macrophage metalloelastase(MMP12) ELISA kit

Mouse Macrophage metalloelastase(MMP12) ELISA kit

  • 中文名稱：
    小鼠巨噬細胞金屬彈力酶(MMP12)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL014659MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠巨噬細胞金屬彈力酶(MMP12)酶聯(lián)免疫試劑盒（CSB-EL014659MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、cellculture勻漿樣本中的MMP12含量。MMP12即基質(zhì)金屬蛋白酶12，在多種生理病理過程中發(fā)揮作用。其可降解細胞外基質(zhì)成分，與組織重塑等密切相關(guān)。在研究中，它常作為靶點探索疾病機制，比如與肺部炎癥、腫瘤侵襲轉(zhuǎn)移等的關(guān)聯(lián)，有望為相關(guān)疾病治療提供新策略。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外研究小鼠模型中MMP12的表達調(diào)控及其在炎癥微環(huán)境、纖維化疾病或腫瘤微環(huán)境中的作用機制分析，支持科研人員對生物樣本中MMP12的精準(zhǔn)定量分析，為探索相關(guān)疾病的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Mmp12 ELISA Kit; Mme ELISA Kit; Mmel ELISA Kit; Macrophage metalloelastase ELISA Kit; MME ELISA Kit; EC 3.4.24.65 ELISA Kit; Matrix metalloproteinase-12 ELISA Kit; MMP-12 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %87
    Range %83-96
    1:200Average %98
    Range %92-105
    1:400Average %102
    Range %98-108
    1:800Average %94
    Range %89-98
  • 回收率：
    The recovery of mouse MMP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-104
    EDTA plasma (n=4)9187-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.801 2.722 2.762 2.608
    2502.110 2.214 2.162 2.008
    1251.427 1.448 1.438 1.284
    62.50.856 0.867 0.862 0.708
    31.20.523 0.512 0.518 0.364
    15.60.351 0.341 0.346 0.192
    7.80.264 0.260 0.262 0.108
    00.154 0.153 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • 基因功能參考文獻：
    1. MMP-12 facilitates macrophage accumulation and activation in anti-GBM glomerulonephritis which is required for glomerular crescent formation, Bowman's capsule rupture, tubular damage and renal function decline. PMID: 27862656
    2. findings define a novel physiological function of Shp2 in TGF-beta1/MMP12-dependent emphysema, adding insights into potential etiologies for this chronic lung disorder. PMID: 28814604
    3. Mmp12 is expressed early following corneal epithelial injury with highest expression levels at 8 h after injury and lower expression levels at 4 and 8 days after injury. We investigated whether MMP12 has an effect on the rate of epithelial repair and cell migration using in vivo and in vitro scratch assays performed on WT and Mmp12(-/-) mice PMID: 28442300
    4. MMP-12 produced by macrophages infiltrating into glomeruli contributed to the degradation of collagen type IV and fibronectin. PMID: 26822129
    5. MMP12 causes arterial stiffening in mice and suggest that it functions similarly in humans. PMID: 26608672
    6. These results suggest that MMP-12 production during emphysema exacerbation results in increased mortality and disease progression. PMID: 26563237
    7. study found significant increases of MMP-3 and MMP-12 mRNA levels and MMP12 zymographic activities in response to Cryptococcus neoformans infection but not C. gattii infection PMID: 26445891
    8. The results suggest that pulmonary C fiber involvement in long-term airway inflammation and airway hyperresponsiveness occurred at least partially via modulating MMP-12, and the activation of PAR2 might be related to MMP-12 production. PMID: 26676790
    9. MMP-12 up-regulation mediated by SARM-TRIF signaling pathway contributes to IFN-gamma-independent airway inflammation and AHR post RSV infection in nude mice. PMID: 25652021
    10. MMP-12 and MMP-13 alter strain K infection in mice and play a role in inflammatory regulation by modulating cytokine levels. PMID: 26438797
    11. Post-MI, MMP-12i impaired CD44-HA interactions to suppress neutrophil apoptosis and prolong inflammation, which worsened LV function. PMID: 25797678
    12. MMP12 upregulated in regenerating motor neurons of the mouse and rat facial nucleus; findings point to a role of MMP12 in the neuronal initiation of the synaptic stripping process PMID: 24907602
    13. abnormal neuronal expression of matrix metalloproteinase-12 may contribute to axonal degeneration in Niemann-Pick type C disease PMID: 25864931
    14. the results of this study show a close correlation between vascular injury markers, Arg 1+ macrophage accumulation and fibrosis and suggest an important role of MMP-12 in regulating these processes. PMID: 25302498
    15. A novel systemic role for MMP12 in postnatal adipose tissue expansion and smoking-associated weight loss by suppressing vascularity within the white adipose tissue depots. PMID: 24914890
    16. Our results suggest that MMP12 released by macrophages activated on dehydrated airway surfaces may play an important role in emphysema formation in the absence of cigarette smoke exposure. PMID: 24828142
    17. Macrophage MMP12 regulates adipose tissue expansion, insulin sensitivity, and expression of inducible nitric oxide synthase. PMID: 24914938
    18. Gene expression of Mmp-12 and Mmp-13, and Timp-1 was strongly upregulated at all time points in RD compared with controls. Timp-2, Mmp-2, and Mmp-9 expression was modest. PMID: 24526442
    19. MMP12 has a protective effect on corneal fibrosis during wound repair through regulation of immune cell infiltration and angiogenesis. PMID: 23813962
    20. Ethanol treatment increases MMP-12 expression in murine peritoneal macrophages RAW 264.7 macrophages. PMID: 23978445
    21. Thus, our study suggested that the upregulated cerebral MMP-12 during aging enhances aging-associated neuroinflammation by facilitating recruitment of bone marrow-derived microglia into the brain. PMID: 23159549
    22. MMP-12 is a key regulator of macrophage infiltration and inflammation, contributing to retinal vascular dysfunction and pathological angiogenesis. PMID: 23285156
    23. Results suggest macrophage-derived MMP-12 contributes to angiostasis in the ischemic lung. PMID: 23061826
    24. MMP-12 does not seem to be essential for macrophage infiltration in adipose tissue, but contributes to the formation of crown-like structures crown-like structures PMID: 23295969
    25. the endogenous matrix metalloprotease-12 active form can be detected with a novel broad spectrum activity-based probe PMID: 23271741
    26. Tanshinone IIA inhibits cell proliferation and TF and MMP-12 expressions in RAW264.7 cells stimulated by ox-LDL. PMID: 19620042
    27. Pneumocystis murina exposure induced Mmp12 mRNA expression in whole lungs and alveolar macrophages in mice. PMID: 22773692
    28. analysis of orally potent and selective small molecule inhibitor of MMP-12 for treatment of COPD and asthma in human and mouse models PMID: 22153340
    29. Macrophage-derived MMP-12 regulates elastin degradation even in progressive experimental liver fibrosis. PMID: 22223197
    30. Provide in vivo evidence of an effect of MMP12 on fibrinogen level. PMID: 22119538
    31. Macrophage metalloelastase (MME) inversely correlates with the expression of bFGF and tumor angiogenesis in a model of murine colon cancer. PMID: 21814804
    32. MMP-12 leads to elastin degradation in eosinophilic meningitis caused by Angiostrongylus cantonensis. PMID: 21856305
    33. MMP12-induced myeloid cell autonomous defect led to abnormal myelopoiesis, immune suppression, and lung adenocarcinoma PMID: 21378275
    34. Selective MMP-12 inhibitor retards atherosclerosis development and results in a more fibrous plaque phenotype in mice. PMID: 21212406
    35. These results suggest that experimental autoimmune encephalomyelitis disease activity may be modulated by the cleavage of osteopontin by matrix metalloproteinase-12. PMID: 20651245
    36. This study examined the hypothesis that the control of NADPH oxidase-2 (Nox2)-mediated reactive oxygen species (ROS) regulates the expression of matrix metalloproteinases (MMPs) and the migration of macrophages. PMID: 19935767
    37. these data suggest important counter-regulatory roles between MMP12 and ECM-degrading enzymes like MMP2, MMP9, and MMP13 in Th2 cytokine-driven fibrosis. PMID: 20181883
    38. MMp12 expression correlates with virulence following neurotropic mouse hepatitis virus infection PMID: 12097550
    39. the role of matrix metalloproteinases-12 in IL-13-induced inflammation and remodeling in the lung PMID: 12189240
    40. conclude that acute smoke-induced connective tissue breakdown, the precursor to emphysema, requires both neutrophils and macrophage metalloelastase PMID: 12204900
    41. Migration of monocytes/macrophages in vitro and in vivo is accompanied by MMP12-dependent tunnel formation and by neovascularization. PMID: 12858542
    42. Increased MMP-12 expression after spinal cord trauma is deleterious and contributes to the development of secondary injury PMID: 14602826
    43. osteoclasts are able to express MMP-12, but MMP-12 did not appear critical for osteoclast recruitment or resorption PMID: 14751561
    44. MMP-12 deficiency conferred significant protection against transmedial elastin degradation and ectasia in the atherosclerotic media. MMP-12 is not involved in atherosclerotic plaque growth. PMID: 14993123
    45. Oligodendrocytes express MMP-12 during their maturation. MMP-12 has functional involvement both in maturation of oligodendrocyte precursor cells & in the ability of OPCs and OLs to extend processes. PMID: 15342725
    46. MMP-12 deficiency attenuates abdominal aortic aneurysmgrowth, possibly by decreasing macrophage recruitment. PMID: 15800495
    47. SP-D influences innate host defense, in part, by regulating sCD14 in a process mediated by MMP-9 and MMP-12. PMID: 15814723
    48. NADPH oxidase restrains the MMP-12 activity of macrophages PMID: 15983040
    49. MMP-12 has a role in inflammation and in T effector polarization PMID: 16081501
    50. Asbestos causes significant increases in mRNA levels of lung matrix metalloproteinases (MMPs 12 and 13) and tissue inhibitor of metalloproteinases (TIMP1), as well as increased activities of MMP 2, 9, and 12 in BAL fluid. PMID: 16571779

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:17381

    STRING: 10090.ENSMUSP00000005950

    UniGene: Mm.2055