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  4. Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

  • 中文名稱：
    小鼠N端前腦鈉素(NT-proBNP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05153m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠N端前腦鈉素(NT-proBNP)酶聯(lián)免疫試劑盒（CSB-E05153m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的NT-proBNP含量。NT-proBNP即N末端B型利鈉肽原，是心室肌細(xì)胞合成B型利鈉肽過(guò)程中裂解產(chǎn)生的無(wú)活性片段。它在心室容量負(fù)荷增加、室壁張力增大時(shí)釋放增加。臨床常通過(guò)檢測(cè)其水平，輔助診斷心力衰竭、評(píng)估病情嚴(yán)重程度及預(yù)后。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于心臟功能相關(guān)研究；在心血管疾病模型構(gòu)建、藥物干預(yù)效果評(píng)估等科研領(lǐng)域具有重要應(yīng)用價(jià)值；適用于心血管基礎(chǔ)研究、轉(zhuǎn)基因動(dòng)物模型評(píng)估、心肌損傷機(jī)制探索等科研場(chǎng)景；可為心衰模型、心肌肥厚等實(shí)驗(yàn)研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    NT-proBNP
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-96
    1:2 Average % 96
    Range % 91-105
    1:4 Average % 89
    Range % 85-99
    1:8 Average % 93
    Range % 83-98
  • 回收率：
    The recovery of mouse NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-105
    EDTA plasma (n=4) 95 91-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.235 2.199 2.217 2.090
    5 1.884 1.749 1.817 1.690
    2.5 1.462 1.375 1.419 1.292
    1.25 0.978 1.035 1.007 0.880
    0.625 0.552 0.549 0.551 0.424
    0.312 0.289 0.284 0.287 0.160
    0.156 0.203 0.211 0.207 0.080
    0 0.128 0.125 0.127 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:


1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, what is the host.
7. Please let us know whether the antibody and the standard include any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

A:
Thanks for your inquiry.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody, affinity chromatography.
Stop solution is 4N sulfuric acid.
Standard is 293 cell-derived recombinant protein）.
The antibody and the standard contain Preservative：proclin 300(1:3000).
The kit doesn't contain thimerosal or sodium azide or 2-mercaptoethanol etc.
Pls let me know if you have any further questions. Thank you.