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  4. Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

  • 中文名稱：
    大鼠N端前腦鈉素(NT-proBNP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08752r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠N端前腦鈉素(NT-proBNP)酶聯(lián)免疫試劑盒（CSB-E08752r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NT-proBNP含量。NT-proBNP是腦鈉肽原裂解后無活性的N末端片段。其分泌受心室壁張力和容量負荷影響。臨床上常作為心力衰竭診斷和預后評估的重要生物標志物，通過檢測血液中水平，反映心臟功能狀態(tài)，輔助判斷病情嚴重程度與指導治療。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于心血管疾病機制研究、心肌損傷動物模型評估及藥物干預效果分析等科研領域；支持對大鼠心臟組織或循環(huán)系統(tǒng)中的NT - proBNP水平進行動態(tài)監(jiān)測，為心肌肥厚、心力衰竭等模型的病理生理研究提供可靠工具，亦可用于探索心血管相關藥物對心室功能的調節(jié)作用。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫：
    NT-proBNP
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-95
    1:2Average %96
    Range %90-101
    1:4Average %99
    Range %94-105
    1:8Average %84
    Range %80-88
  • 回收率：
    The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9489-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.769 2.651 2.710 2.548
    2501.973 1.901 1.937 1.775
    1251.183 1.217 1.200 1.038
    62.50.793 0.841 0.817 0.655
    31.20.506 0.550 0.528 0.366
    15.60.404 0.379 0.392 0.230
    7.80.261 0.245 0.253 0.091
    00.165 0.159 0.162 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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