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  4. Mouse Procalcitonin,PCT ELISA Kit

Mouse Procalcitonin,PCT ELISA Kit

  • 中文名稱：
    小鼠降鈣素原(PCT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E10371m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E10371m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PCT含量。降鈣素原（PCT）是一種蛋白質(zhì)。在健康人體中，PCT 水平極低。當(dāng)發(fā)生嚴(yán)重細(xì)菌、真菌、寄生蟲(chóng)感染及膿毒癥和多臟器功能衰竭時(shí)，其水平會(huì)顯著升高。研究認(rèn)為，它可作為炎癥和感染的生物標(biāo)志物，輔助醫(yī)生判斷病情和指導(dǎo)治療。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，可應(yīng)用于科研場(chǎng)景中PCT相關(guān)機(jī)制研究?？蒲腥藛T可將其應(yīng)用于實(shí)驗(yàn)動(dòng)物模型中膿毒癥、全身性感染及炎癥性疾病的機(jī)制探索，或用于評(píng)估藥物干預(yù)對(duì)炎癥通路的影響。本試劑盒支持多種生物樣本類型檢測(cè)，為實(shí)驗(yàn)室開(kāi)展PCT動(dòng)態(tài)監(jiān)測(cè)、病原體 - 宿主互作研究及免疫調(diào)節(jié)實(shí)驗(yàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    PCT
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %91
    Range %88-93
    1:40Average %102
    Range %99-104
    1:80Average %94
    Range %89-96
    1:160Average %97
    Range %94-101
  • 回收率：
    The recovery of mouse PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9690-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.224 2.304 2.264 2.090
    501.655 1.685 1.670 1.496
    251.122 1.157 1.140 0.966
    12.50.732 0.721 0.727 0.553
    6.250.441 0.424 0.433 0.259
    3.120.315 0.302 0.309 0.135
    1.560.242 0.228 0.235 0.061
    00.176 0.172 0.174 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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