Pig Procalcitonin,PCT ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E15004p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PCT含量。PCT（降鈣素原）是一種蛋白質(zhì)。它健康狀態(tài)下在體內(nèi)水平極低，感染時(shí)會(huì)顯著升高。其研究機(jī)制主要圍繞作為感染診斷和病情監(jiān)測(cè)的生物標(biāo)志物，在細(xì)菌感染時(shí)，機(jī)體產(chǎn)生炎癥反應(yīng)促使PCT合成增加，可輔助判斷感染嚴(yán)重程度與預(yù)后。試劑盒檢測(cè)范圍為39 pg/mL-2500 pg/mL，適用于科研場(chǎng)景中豬類(lèi)疾病模型的炎癥進(jìn)程監(jiān)測(cè)、疫苗或藥物干預(yù)效果評(píng)估、免疫調(diào)控機(jī)制研究等方向，為獸醫(yī)科學(xué)、動(dòng)物醫(yī)學(xué)研究及生物標(biāo)志物開(kāi)發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

PCT
種屬：

Sus scrofa (Pig)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	95
	Range %	88-98
1:4	Average %	92
	Range %	89-96
1:8	Average %	93
	Range %	88-98

回收率：

The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	96	92-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.