搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse Roundabout homolog 4(ROBO4) ELISA kit

Mouse Roundabout homolog 4(ROBO4) ELISA kit

  • 中文名稱：
    小鼠環(huán)形交叉軸突導(dǎo)向受體同源物4(ROBO4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020057MO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠環(huán)形交叉軸突導(dǎo)向受體同源物4(ROBO4)酶聯(lián)免疫試劑盒（CSB-EL020057MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ROBO4含量。ROBO4是一個重要靶點。其在血管生成與穩(wěn)定性維持等方面具關(guān)鍵作用。研究機制上，它能參與調(diào)節(jié)內(nèi)皮細胞的行為和功能，影響血管的發(fā)育和重塑，在腫瘤血管生成等病理過程的研究中受到關(guān)注，或為相關(guān)疾病治療提供新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，廣泛用于血管生物學(xué)、腫瘤微環(huán)境、缺血性疾病及炎癥相關(guān)機制的基礎(chǔ)研究，為探索內(nèi)皮細胞功能調(diào)控及病理生理過程提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Robo4 ELISA Kit; Roundabout homolog 4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 86    
    Range % 81-89    
    1:2 Average % 98    
    Range % 94-103    
    1:4 Average % 86    
    Range % 83-89    
    1:8 Average % 98    
    Range % 95-102    
  • 回收率：
    The recovery of mouse ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 101-109    
    EDTA plasma (n=4) 89 85-93    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 2.524 2.631 2.578 2.404    
    5 1.957 1.904 1.931 1.757    
    2.5 1.240 1.304 1.272 1.098    
    1.25 0.753 0.768 0.761 0.587    
    0.625 0.496 0.511 0.504 0.330    
    0.312 0.302 0.320 0.311 0.137    
    0.156 0.220 0.234 0.227 0.053    
    0 0.176 0.172 0.174 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function.
  • 基因功能參考文獻：
    1. Robo4 inhibits angiogenesis and vessel permeability independently of its cytoplasmic domain. Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing. PMID: 27882935
    2. endothelial Robo4 signaling is important for suppressing breast cancer growth and metastasis, and it can be targeted (enhanced) by administrating a small molecular drug. PMID: 26778715
    3. Robo4 vaccines induce antibodies that retard tumor growth. PMID: 25348086
    4. Robo4 and Src family kinases are involved in the mechanisms of embryonic vascular defects caused by the Hrt/Hey deficiency. PMID: 25264302
    5. These results clearly indicate that Robo4 plays a role in hematopoietic stem cells trafficking such as bone marrow homing and peripheral mobilization. PMID: 23226403
    6. our results showed an important role of Robo4 in the regulation of cortical radial migration through Slit-dependent and -independent mechanisms PMID: 22123939
    7. Data found that expression of Slit2 and SDF1 were dynamically regulated in mesenchymal stem cells and OBC-like populations following radiation, while Robo4 expression was restricted to endothelial cells. PMID: 22313734
    8. augmenting the host Robo4/Slit2 system could provide a useful therapeutic approach to control pathological angiogenesis associated with HSV induced stromal keratitis PMID: 21572022
    9. Robo4 specifically binds to UNC5B, a vascular Netrin receptor, revealing unexpected interactions between two endothelial guidance receptors. PMID: 21238923
    10. Activating with the soluble ligand Slit an endothelium-specific, Robo4-dependent signaling pathway that strengthens the vascular barrier, diminishing deleterious aspects of the host's response to the pathogen-induced cytokine storm. PMID: 20375003
    11. enhanced growth of blood vessels in Robo4(-/-) endothelium is due to activation of vascular endothelial growth factor (VEGF)-R2 signaling through the Src and FAK kinases. PMID: 20498081
    12. Robo4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. PMID: 12941633
    13. Robo4 is expressed in hematopoietic stem cells (HSC), and Robo4/Slit2 signaling may play a role in HSC homeostasis in the bone marrow niche. PMID: 18927479
    14. Robo4 mRNA level decreases during vascular decline in pulmonary fibrosis. PMID: 17496152

    顯示更多

    收起更多

  • 蛋白家族：
    Immunoglobulin superfamily, ROBO family
  • 組織特異性：
    Expressed specifically in embryo and adult vascular endothelium.
  • 數(shù)據(jù)庫鏈接：