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  4. Mouse Substance P,SP ELISA Kit

Mouse Substance P,SP ELISA Kit

  • 中文名稱：
    小鼠P物質(zhì)(SP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08359m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠P物質(zhì)(SP)酶聯(lián)免疫試劑盒（CSB-E08359m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SP含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，廣泛用于基礎(chǔ)科研領(lǐng)域，如探究神經(jīng)系統(tǒng)疾病模型中SP的分泌動態(tài)、評估藥物對神經(jīng)肽表達(dá)的調(diào)控作用，或解析炎癥反應(yīng)與神經(jīng)信號通路的關(guān)聯(lián)機(jī)制，為神經(jīng)生物學(xué)、免疫學(xué)及藥理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    SP
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %96
    Range %92-104
    1:200Average %94
    Range %89-98
    1:400Average %89
    Range %86-96
    1:800Average %94
    Range %89-97
  • 回收率：
    The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8682-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.615 2.514 2.565 2.381
    1001.887 1.786 1.837 1.653
    501.093 1.073 1.083 0.899
    250.668 0.657 0.663 0.479
    12.50.436 0.447 0.442 0.258
    6.250.311 0.325 0.318 0.134
    3.120.241 0.246 0.244 0.060
    00.180 0.187 0.184 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:200

產(chǎn)品評價： 我用CSB-E08359m檢測正常血清，其od值為0.7/0.604/0.559/0.639/0.577/0.671

By 張老師