Rat Substance P,SP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠P物質(zhì)(SP)酶聯(lián)免疫試劑盒（CSB-E08358r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SP含量。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，其高特異性與穩(wěn)定性可滿足科研領(lǐng)域?qū)ι窠?jīng)生物學(xué)機(jī)制、炎癥相關(guān)疾病模型或胃腸道功能研究的檢測(cè)需求，例如通過(guò)分析不同實(shí)驗(yàn)條件下動(dòng)物模型血清或組織中的SP表達(dá)水平，探索其在神經(jīng)信號(hào)傳導(dǎo)或免疫調(diào)節(jié)中的作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

SP
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	98-106
1:4	Average %	92
	Range %	87-95
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	91-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.