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  4. Mouse Thrombopoietin,TPO ELISA kit

Mouse Thrombopoietin,TPO ELISA kit

  • 中文名稱：
    小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04746m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒（CSB-E04746m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的THPO含量。THPO即血小板生成素，作為調(diào)節(jié)血小板生成的關(guān)鍵因子，具有刺激巨核細(xì)胞生長(zhǎng)、分化和促進(jìn)血小板生成的重要作用。其研究機(jī)制主要圍繞與受體結(jié)合激活信號(hào)通路展開，為血小板減少癥等相關(guān)疾病的治療藥物研發(fā)提供了重要靶點(diǎn)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于探究TPO在血液系統(tǒng)疾病模型中的動(dòng)態(tài)表達(dá)規(guī)律、評(píng)估藥物干預(yù)對(duì)血小板生成的影響，或解析組織微環(huán)境中TPO的調(diào)控機(jī)制等科研領(lǐng)域，為研究血小板生成調(diào)控網(wǎng)絡(luò)提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Thpo ELISA Kit; Thrombopoietin ELISA Kit; C-mpl ligand ELISA Kit; ML ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; MGDF ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    88-100

    1:2

    Average %

    93

    Range %

    88-97

    1:4

    Average %

    93

    Range %

    86-98

    1:8

    Average %

    90

    Range %

    88-96

  • 回收率：

    The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    99

    97-103

    EDTA plasma (n=4)

    102

    97-110

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.540

    2.634

    2.587

    2.409

    200

    1.857

    1.767

    1.812

    1.634

    100

    1.120

    1.128

    1.124

    0.946

    50

    0.734

    0.729

    0.732

    0.553

    25

    0.501

    0.511

    0.506

    0.328

    12.5

    0.369

    0.353

    0.361

    0.183

    6.25

    0.272

    0.284

    0.278

    0.100

    0

    0.182

    0.175

    0.178

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • 基因功能參考文獻(xiàn)：
    1. Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID: 29217821
    2. this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID: 29622652
    3. TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID: 27344013
    4. Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID: 25137252
    5. TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID: 25212676
    6. novel findings about aspects of TPO action on stem cells PMID: 25564715
    7. Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
    8. Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID: 25298035
    9. Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID: 25451253
    10. IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID: 24184684
    11. Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
    12. Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID: 21507308
    13. The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID: 21149592
    14. Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID: 12005542
    15. glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID: 12101178
    16. Thrombopoietin expands hematopoietic stem cells after transplantation PMID: 12163458
    17. p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID: 12855555
    18. Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
    19. Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID: 15254242
    20. mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
    21. is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID: 15642952
    22. TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID: 15665119
    23. Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID: 15927672
    24. Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID: 16022585
    25. results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID: 17284614
    26. thrombopoietin has a role in mast cell differentiation [review] PMID: 17468237
    27. Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID: 17546634
    28. roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID: 17925492
    29. Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID: 18410987
    30. TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID: 18473128
    31. thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID: 18773906
    32. TPO downmodulates c-Myb expression through induction of miR-150. PMID: 18814950

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 組織特異性：
    Found mainly in the liver, kidney and skeletal muscle.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:21832

    STRING: 10090.ENSMUSP00000075756

    UniGene: Mm.3943