Mouse Thromboxane B2,TXB2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血栓素B2(TXB2)酶聯(lián)免疫試劑盒（CSB-E08048m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TXB2含量。TXB2 即血栓素 B2，是血栓素 A2 的穩(wěn)定水解產(chǎn)物。它在體內(nèi)參與血小板聚集和血管收縮過(guò)程。研究機(jī)制方面，常通過(guò)檢測(cè)其水平反映體內(nèi)血栓素 A2 活性，以評(píng)估血小板功能、血管內(nèi)皮損傷及血栓形成傾向，在心血管疾病等研究中受關(guān)注。試劑盒檢測(cè)范圍為2.35 pg/mL-150 pg/mL，其水平變化常被用于評(píng)估血栓形成、心血管疾病及炎癥相關(guān)機(jī)制的研究；適用于心血管疾病模型、炎癥機(jī)制研究、抗血小板藥物篩選等科研領(lǐng)域，可為探索病理生理過(guò)程中TXB2的動(dòng)態(tài)變化及其分子調(diào)控網(wǎng)絡(luò)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

TXB2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

2.35 pg/mL-150 pg/mL
靈敏度：

0.58 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-104
1:2	Average %	89
	Range %	84-94
1:4	Average %	90
	Range %	85-96
1:8	Average %	98
	Range %	93-103

回收率：

The recovery of mouse TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-102
EDTA plasma (n=4)	91	86-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.