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  4. Rat Thromboxane B2,TXB2 ELISA Kit

Rat Thromboxane B2,TXB2 ELISA Kit

  • 中文名稱：
    大鼠血栓素B2(TXB2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08047r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血栓素B2(TXB2)酶聯(lián)免疫試劑盒（CSB-E08047r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TXB2含量。TXB2是一種由TXA2酶促代謝產(chǎn)生的穩(wěn)定水解產(chǎn)物，是TXA2的穩(wěn)定代謝物，可以反映TXA2的全身生物合成速率。TXB2在生理和病理過程中扮演重要角色，如參與止血、動脈粥樣硬化、血栓形成等。研究TXB2有助于了解相關疾病的發(fā)生機制，為疾病診斷和治療提供新的靶點。試劑盒檢測范圍為3.9 pg/mL-250 pg/mL，適用于心血管疾病動物模型研究、抗血小板藥物藥效評估、炎癥相關機制探索等科研領域，滿足基礎研究中對TXB2動態(tài)變化的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    TXB2
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.9 pg/mL-250 pg/mL
  • 靈敏度：
    0.97 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %88-98
    1:2Average %95
    Range %91-102
    1:4Average %99
    Range %94-106
    1:8Average %93
    Range %87-97
  • 回收率：
    The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-103
    EDTA plasma (n=4)9288-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2501.990 1.890 1.940 1.785
    1251.298 1.198 1.248 1.093
    62.50.786 0.766 0.776 0.621
    31.250.545 0.525 0.535 0.380
    15.60.341 0.321 0.331 0.176
    7.80.292 0.272 0.282 0.127
    3.90.215 0.211 0.213 0.058
    00.156 0.154 0.155 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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