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  4. Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

  • 中文名稱：
    小鼠轉(zhuǎn)化生長(zhǎng)因子β3(TGF-β3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12862m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠轉(zhuǎn)化生長(zhǎng)因子β3(TGF-β3)酶聯(lián)免疫試劑盒（CSB-E12862m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TGFB3含量。TGFB3即轉(zhuǎn)化生長(zhǎng)因子β3，在胚胎發(fā)育、組織修復(fù)等多種生理過(guò)程中扮演重要角色。其研究機(jī)制主要圍繞調(diào)節(jié)細(xì)胞生長(zhǎng)、分化、黏附及凋亡等方面。異常表達(dá)與腫瘤發(fā)生發(fā)展、纖維化疾病等密切相關(guān)，對(duì)其深入研究有望為相關(guān)疾病治療提供新策略。試劑盒檢測(cè)范圍為125 pg/mL-8000 pg/mL，本試劑盒適用于體外實(shí)驗(yàn)研究，可廣泛用于探究TGF-β3在細(xì)胞功能調(diào)控、炎癥或纖維化動(dòng)物模型中的動(dòng)態(tài)變化，亦可用于評(píng)估體外培養(yǎng)細(xì)胞分泌TGF-β3的能力，為研究組織再生、疾病機(jī)制及藥物干預(yù)效果提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Tgfb3 ELISA Kit; Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-3 ELISA Kit; TGF-beta-3)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %95
    Range %92-102
    1:4Average %96
    Range %93-102
    1:8Average %94
    Range %89-99
  • 回收率：
    The recovery of mouse TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-99
    EDTA plasma (n=4)9184-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.646 2.815 2.731 2.542
    40002.016 2.155 2.086 1.897
    20001.195 1.264 1.230 1.041
    10000.778 0.798 0.788 0.599
    5000.493 0.506 0.500 0.311
    2500.336 0.361 0.349 0.160
    1250.279 0.265 0.272 0.083
    00.192 0.185 0.189 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.; Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.; Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
  • 亞細(xì)胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-3]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數(shù)據(jù)庫(kù)鏈接：