Mouse calprotectin (CALP) ELISA kit

產(chǎn)品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠鈣衛(wèi)蛋白(CALP)酶聯(lián)免疫試劑盒（CSB-EQ013485MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CALP含量。材料未給出CALP靶點相關背景及研究機制的具體內(nèi)容，無法為你總結。若有更多關于CALP靶點的詳細信息，比如在哪些疾病研究中涉及、作用于機體的大致原理等，請補充后，我再進行總結。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研領域中對炎癥相關動物模型（如結腸炎、敗血癥或關節(jié)炎模型）的病理機制研究，通過定量分析CALP水平，可評估中性粒細胞活化狀態(tài)及組織炎癥程度，為探索疾病發(fā)展、藥物治療效果或生物標志物篩選提供實驗依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CALP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of mouse CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	96	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.