Pig calprotectin (CALP) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬鈣衛(wèi)蛋白(CALP)酶聯(lián)免疫試劑盒（CSB-EQ013485PI）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CALP含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于動(dòng)物疾病模型研究（如腸道炎癥、感染性疾病）、免疫機(jī)制探索或藥物開發(fā)中炎癥標(biāo)志物的動(dòng)態(tài)監(jiān)測(cè)。適用于獸醫(yī)病理學(xué)、比較醫(yī)學(xué)及畜牧養(yǎng)殖領(lǐng)域的科研分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

CALP
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-102
1:200	Average %	85
	Range %	81-89
1:400	Average %	103
	Range %	98-107
1:800	Average %	87
	Range %	83-92

回收率：

The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	106	102-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.