Mouse copeptin ELISA kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠和肽素(copeptin)酶聯(lián)免疫試劑盒（CSB-E14921m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的copeptin含量。copeptin是精氨酸加壓素原的C端片段，穩(wěn)定性好，可反映精氨酸加壓素的釋放水平。在研究機制上，它可作為生物標志物用于評估多種疾病。如在心血管疾病、腎臟疾病等的診斷、病情監(jiān)測及預后評估方面展現(xiàn)出潛在價值。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于基礎科研中探討小鼠模型在應激性疾病、水鹽代謝異常、心血管功能障礙或神經(jīng)內分泌紊亂等生理病理過程中的分子機制，為動物實驗提供高效的分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

copeptin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	96
	Range %	92-102
1:4	Average %	102
	Range %	96-108
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	88	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.