Rat Copeptin ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠和肽素(copeptin)酶聯(lián)免疫試劑盒（CSB-E16335r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的copeptin含量。copeptin是精氨酸加壓素原的C末端片段，穩(wěn)定性好。其背景在于檢測它可間接反映精氨酸加壓素水平。研究機制方面，在一些疾病如心血管病、內分泌疾病等中，copeptin水平會發(fā)生變化，可作為疾病診斷、病情評估和預后判斷的生物標志物。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品適用于實驗室研究中對大鼠模型在病理生理狀態(tài)下的和肽素水平動態(tài)監(jiān)測，例如心血管疾病機制探索、應激反應評估或腎臟功能相關研究，為深入解析相關疾病的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

copeptin
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-102
1:2	Average %	94
	Range %	91-99
1:4	Average %	99
	Range %	96-106
1:8	Average %	97
	Range %	90-102

回收率：

The recovery of rat copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	88	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.