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  4. Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

  • 中文名稱：
    小鼠Ⅰ型前膠原N端前肽(PⅠNP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12775m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠Ⅰ型前膠原N端前肽(PⅠNP)酶聯(lián)免疫試劑盒（CSB-E12775m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅠNP含量。PⅠNP是一種重要靶點。其與骨代謝密切相關(guān)，可反映成骨細胞活性。研究機制上，通過檢測血液中其含量變化，能評估骨形成狀態(tài)，在骨質(zhì)疏松等骨骼疾病的診斷、病情監(jiān)測及治療效果評估等研究中發(fā)揮著關(guān)鍵作用。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，PⅠNP可作為骨形成和纖維化進程的關(guān)鍵生物標(biāo)志物，在骨代謝研究、纖維化疾病模型評估及藥物干預(yù)效果分析中具有重要科研價值。適用于研究骨發(fā)育異常、骨質(zhì)疏松模型、肝/肺纖維化機制探索等科研領(lǐng)域，為相關(guān)疾病模型的生物標(biāo)志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
  • 縮寫：
    PⅠNP
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.7 pg/mL-300 pg/mL
  • 靈敏度：
    1.17 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-98  
    1:200 Average % 95  
    Range % 91-98  
    1:400 Average % 91  
    Range % 85-96  
    1:800 Average % 94  
    Range % 88-102  
  • 回收率：
    The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-99  
    EDTA plasma (n=4) 89 82-94  
                 
                 
  • 標(biāo)準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.413 2.313 2.363 2.261  
    150 1.598 1.498 1.548 1.446  
    75 0.940 0.890 0.915 0.813  
    37.5 0.489 0.479 0.484 0.382  
    18.75 0.316 0.306 0.311 0.209  
    9.4 0.207 0.205 0.206 0.104  
    4.7 0.145 0.142 0.144 0.042  
    0 0.103 0.101 0.102 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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