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  4. Mouse Kallikrein 1-related peptidase b3,Klk3 ELISA Kit

Mouse Kallikrein 1-related peptidase b3,Klk3 ELISA Kit

  • 中文名稱：
    小鼠激肽釋放酶1相關(guān)肽酶b3(KLK3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08276m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠激肽釋放酶1相關(guān)肽酶b3(KLK3)酶聯(lián)免疫試劑盒（CSB-E08276m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KLK3含量。KLK3，即激肽釋放酶相關(guān)肽酶3，是一種由前列腺上皮細胞分泌的糖蛋白，屬于激肽釋放酶相關(guān)肽酶家族。KLK3在前列腺癌的發(fā)生發(fā)展中扮演重要角色，其表達水平與前列腺癌的侵襲性相關(guān)。研究顯示，KLK3基因突變可以預(yù)測Gleason評分為7分的前列腺癌患者的侵襲性，為前列腺癌的診斷和治療提供了新的靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品可廣泛應(yīng)用于小鼠模型的基礎(chǔ)研究領(lǐng)域，例如通過檢測血清或組織中的 KLK3 表達水平，探索其在前列腺疾病、腫瘤進展或炎癥反應(yīng)中的作用機制，亦可用于藥物干預(yù)實驗中的生物標(biāo)志物動態(tài)監(jiān)測，為科研人員提供穩(wěn)定、高效的體外檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-100  
    1:2 Average % 93  
    Range % 88-97  
    1:4 Average % 89  
    Range % 84-95  
    1:8 Average % 104  
    Range % 100-108  
  • 回收率：
    The recovery of mouse KLK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.474 2.352 2.413 2.242  
    10 2.004 2.076 2.040 1.869  
    5 1.425 1.354 1.390 1.219  
    2.5 0.764 0.801 0.783 0.612  
    1.25 0.482 0.512 0.497 0.326  
    0.625 0.350 0.336 0.343 0.172  
    0.312 0.243 0.235 0.239 0.068  
    0 0.169 0.173 0.171    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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