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  4. Mouse secretory immunoglobulin A,sIgA ELISA Kit

Mouse secretory immunoglobulin A,sIgA ELISA Kit

  • 中文名稱：
    小鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08413m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E08413m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、支氣管肺泡灌洗液樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫系統(tǒng)的主要效應(yīng)分子。能夠在黏膜表面阻止病原體黏附與入侵，發(fā)揮抗感染等作用。研究機(jī)制主要圍繞其生成、分泌過程，以及與病原體相互作用等方面，以探索其在疾病防治中的應(yīng)用潛力。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于科研場景中黏膜免疫相關(guān)動(dòng)物模型的機(jī)制研究，例如呼吸道感染模型中局部免疫應(yīng)答的動(dòng)態(tài)監(jiān)測、腸道炎癥疾病中黏膜屏障功能的評估，或藥物/益生菌干預(yù)后sIgA分泌水平的定量分析。其寬泛的樣本兼容性可支持多維度實(shí)驗(yàn)設(shè)計(jì)，尤其適用于支氣管肺泡灌洗液等低濃度樣本的檢測需求，為黏膜免疫調(diào)控機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    sIgA
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %92
    Range %87-95
    1:40Average %101
    Range %97-104
    1:80Average %93
    Range %87-97
    1:160Average %98
    Range %92-102
  • 回收率：
    The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9692-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.486 2.466 2.476 2.312
    5001.772 1.841 1.807 1.643
    2501.232 1.222 1.227 1.063
    1250.760 0.748 0.754 0.590
    62.50.464 0.481 0.473 0.309
    31.20.325 0.312 0.319 0.155
    15.60.239 0.229 0.234 0.070
    00.166 0.162 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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