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  4. Pig secretory immunoglobulin A,SIgA ELISA Kit

Pig secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名稱：
    豬分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12063p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E12063p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫的主要效應(yīng)分子。其能在黏膜表面阻止病原體黏附與入侵，中和毒素等。研究中聚焦于其產(chǎn)生機(jī)制、影響因素等，以探索增強(qiáng)sIgA水平或功能的方法，用于防治黏膜感染等疾病。試劑盒檢測(cè)范圍為0.15 μg/mL-37.5 μg/mL，適用于動(dòng)物免疫機(jī)制研究，如評(píng)估腸道感染模型中的黏膜免疫應(yīng)答、疫苗效價(jià)驗(yàn)證或飼料添加劑對(duì)免疫調(diào)節(jié)的影響等領(lǐng)域。支持科研人員在體外實(shí)驗(yàn)中系統(tǒng)性分析sIgA的動(dòng)態(tài)變化，為豬類免疫學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    sIgA
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.15 μg/mL-37.5 μg/mL
  • 靈敏度：
    0.15 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 88-97  
    1:400 Average % 94  
    Range % 90-98  
    1:800 Average % 87  
    Range % 85-94  
    1:1600 Average % 96  
    Range % 91-103  
  • 回收率：
    The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 103 98-108  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    37.5 0.120 0.115 0.118    
    9.38 0.456 0.463 0.460    
    2.34 0.901 0.877 0.889    
    0.58 1.432 1.527 1.480    
    0.15 2.103 2.189 2.146    
    0 2.544 2.753 2.649    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：其他 豬

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 標(biāo)曲準(zhǔn)確，樣本數(shù)值在標(biāo)曲范圍之內(nèi)，符合預(yù)期結(jié)果。

By 謝老師

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：其他 豬

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 我用Pig secretory immunoglobulin A (SIgA) ELISA Kit檢測(cè)細(xì)胞培養(yǎng)上清中的SIgA含量 操作步驟很簡(jiǎn)單 耗時(shí)短 最終測(cè)得的含量也很合理

By 劉老師

相關(guān)問答

■ 常見問題解答
Q:

Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?

A:
Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.