Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒（CSB-E08710m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TCC C5b9含量。小鼠末端補(bǔ)體復(fù)合物C5b-9（TCC C5b-9）是補(bǔ)體系統(tǒng)激活終末階段的產(chǎn)物，通過介導(dǎo)細(xì)胞膜穿孔參與免疫防御和炎癥反應(yīng)調(diào)控。其在膜性腎病等免疫相關(guān)疾病中發(fā)揮重要作用，研究其機(jī)制有助于揭示疾病發(fā)病機(jī)制，為疾病治療提供新靶點(diǎn)。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，該產(chǎn)品適用于科研場景中炎癥性疾病模型評估、補(bǔ)體通路功能研究或藥物干預(yù)效果分析，為探索免疫調(diào)節(jié)機(jī)制提供可靠工具，所有應(yīng)用僅限于實(shí)驗室研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TCC C5b9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.