Rat terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠末端補體復合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒（CSB-E08709r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TCC C5b9含量。大鼠末端補體復合物C5b-9（TCC C5b-9）是補體系統(tǒng)激活后形成的膜攻擊復合物，通過介導細胞膜穿孔參與免疫防御及炎癥反應調(diào)控，在免疫相關疾病機制研究中具有重要價值。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于基礎醫(yī)學研究中補體系統(tǒng)功能評估，例如在建立腎小球腎炎、心肌梗死或神經(jīng)退行性疾病動物模型時，可通過檢測血清或組織中的C5b - 9水平動態(tài)觀察補體激活程度；亦可用于評價藥物或生物制劑對補體通路的調(diào)控作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TCC C5b9
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	96
	Range %	92-102
1:400	Average %	85
	Range %	81-88
1:800	Average %	88
	Range %	83-95

回收率：

The recovery of rat TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	87-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.