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  4. Pig Erythropoietin,EPO ELISA Kit

Pig Erythropoietin,EPO ELISA Kit

  • 中文名稱：
    豬紅細(xì)胞生成素(EPO)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12056p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4500/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬紅細(xì)胞生成素(EPO)酶聯(lián)免疫試劑盒（CSB-E12056p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EPO含量。EPO（促紅細(xì)胞生成素）是一種糖蛋白激素，主要在腎臟和肝臟產(chǎn)生，控制紅細(xì)胞的生成。其研究機(jī)制涉及調(diào)節(jié)紅細(xì)胞前體細(xì)胞的分化，并參與神經(jīng)保護(hù)和傷口愈合等過(guò)程。EPO在治療貧血和某些疾病中發(fā)揮重要作用，但其副作用和興奮劑使用也備受關(guān)注。試劑盒檢測(cè)范圍為1 mIU/mL-40 mIU/mL，適用于動(dòng)物生理機(jī)制研究、疾病模型構(gòu)建、藥物干預(yù)效果評(píng)估等科研領(lǐng)域，尤其為豬的血液系統(tǒng)疾病研究、腎臟功能探索及缺氧適應(yīng)性分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    EPOErythropoietin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1 mIU/mL-40 mIU/mL
  • 靈敏度：
    0.5 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 83-91  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 97  
    Range % 95-101  
    1:8 Average % 100  
    Range % 97-103  
  • 回收率：
    The recovery of pig EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 95-102  
    EDTA plasma (n=4) 93 90-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml. OD1 OD2 Average    
    40 2.687 2.699 2.693    
    20 1.980 1.879 1.930    
    10 1.105 1.097 1.101    
    5 0.604 0.634 0.619    
    2 0.271 0.293 0.282    
    1 0.132 0.134 0.133    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Could you please advise on the following: 1) what sample types can be used? (plasma, serum, etc)
2) has the kit been validated using porcine samples?
3) was it native or recombinant protein that was tested? Thank you

A:
Thanks for your inquiry.
We tested pig serum and plasma samples before and the test value is
ND--2mIU/ml. Most samples can not be detected out.
Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能參考文獻(xiàn)：
    1. EPO might play a role as a survival factor or as a mitogen in developing cartilage tissue. PMID: 19908127
    2. Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass. PMID: 19767525
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 組織特異性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 數(shù)據(jù)庫(kù)鏈接：