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  4. Pig Haptoglobin,Hpt/HP ELISA Kit

Pig Haptoglobin,Hpt/HP ELISA Kit

  • 中文名稱：
    豬結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13424p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)酶聯(lián)免疫試劑盒（CSB-E13424p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HP含量。試劑盒檢測范圍為1.56 μg/mL-100 μg/mL，其應(yīng)用場景涵蓋動(dòng)物醫(yī)學(xué)研究中的炎癥標(biāo)志物動(dòng)態(tài)監(jiān)測、疾病模型建立效果評(píng)估，以及畜牧養(yǎng)殖中豬群健康狀態(tài)的分子水平研究，為探索Hpt/HP在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 μg/mL-100 μg/mL
  • 靈敏度：
    0.39 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 89-98  
    1:40 Average % 98  
    Range % 93-103  
    1:80 Average % 95  
    Range % 89-98  
    1:160 Average % 94  
    Range % 87-98  
  • 回收率：
    The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 87 82-94  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ug/ml OD1 OD2 Average Corrected  
    100 1.812 1.724 1.768 1.625  
    50 1.145 1.212 1.179 1.036  
    25 0.715 0.724 0.720 0.577  
    12.5 0.451 0.438 0.445 0.302  
    6.25 0.312 0.322 0.317 0.174  
    3.12 0.255 0.242 0.249 0.106  
    1.56 0.196 0.190 0.193 0.050  
    0 0.142 0.144 0.143    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
  • 基因功能參考文獻(xiàn)：
    1. crystal structure of the dimeric porcine haptoglobin-haemoglobin complex determined at 2.9 A resolution PMID: 22922649
    2. This protein is elevated in porcine postweaning multisystemic wasting syndrome. PMID: 15210076
    3. haptoglobin has a role in oxidative status in piglets undergoing weaning-induced stress PMID: 16438801
    4. This study suggested closer links between the release of haptoglobin, Pig-MAP and monocytes compared to the release of AGP, SAA and transthyretin. PMID: 17629575
    5. The haptoglobin was down-regulated with SNPs PMID: 18777092
    6. Presence of haptoglobin alpha 1S subunit is a reponse to respiratory syndrome virus infection. PMID: 18931071

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:397061

    STRING: 9823.ENSSSCP00000002967

    UniGene: Ssc.37