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  4. Pig Interleukin 1α,IL-1α ELISA kit

Pig Interleukin 1α,IL-1α ELISA kit

  • 中文名稱：
    豬白介素1α(IL-1α)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09725p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素1α(IL-1α)酶聯(lián)免疫試劑盒（CSB-E09725p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1A含量。IL1A是重要的細胞因子，在炎癥反應(yīng)等生理病理過程中發(fā)揮關(guān)鍵作用。其與多種炎癥及免疫相關(guān)疾病關(guān)聯(lián)。研究機制上，它主要通過與相應(yīng)受體結(jié)合，激活下游信號通路，從而調(diào)節(jié)免疫細胞功能、介導(dǎo)炎癥反應(yīng)，是當(dāng)前熱門研究靶點。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，可為豬類動物模型中的炎癥機制研究、疫苗開發(fā)評估、疾病進程監(jiān)測等科研項目提供可靠數(shù)據(jù)支持，例如在豬繁殖與呼吸綜合征、豬流行性腹瀉等感染性疾病模型中定量分析IL - 1α的動態(tài)變化，或在免疫調(diào)控實驗中評估細胞因子網(wǎng)絡(luò)的作用機制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %82-93
    1:2Average %104
    Range %100-108
    1:4Average %91
    Range %85-97
    1:8Average %87
    Range %83-92
  • 回收率：
    The recovery of pig IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9995-103
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.599 2.535 2.567 2.385
    8001.839 1.899 1.869 1.687
    4001.174 1.197 1.186 1.004
    2000.753 0.742 0.748 0.566
    1000.460 0.499 0.480 0.298
    500.368 0.372 0.370 0.188
    250.284 0.292 0.288 0.106
    00.181 0.183 0.182
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能參考文獻：
    1. Constitutive suppression of IL-1alpha maintains quiescence of endothelium and that terminal complement complexes remove that suppression, allowing IL-1alpha transcription and, ultimately, activation of endothelium to proceed. PMID: 18292602
  • 亞細胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:397094

    STRING: 9823.ENSSSCP00000008638

    UniGene: Ssc.113