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  4. Pig Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

Pig Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

  • 中文名稱：
    豬清道夫受體富含半胱氨酸1蛋白M130(CD163)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL004882PI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬清道夫受體富含半胱氨酸1蛋白M130(CD163)酶聯(lián)免疫試劑盒（CSB-EL004882PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CD163含量。CD163是一種細(xì)胞表面糖蛋白，在單核細(xì)胞和巨噬細(xì)胞上表達(dá)。其可被特定分子識(shí)別激活，在炎癥反應(yīng)、組織修復(fù)等生理過程中發(fā)揮重要作用。目前研究聚焦于其作為炎癥標(biāo)志物及治療靶點(diǎn)，探索調(diào)節(jié)其功能來(lái)干預(yù)相關(guān)疾病。試劑盒檢測(cè)范圍為93.75 ng/mL-6000 ng/mL，該產(chǎn)品適用于動(dòng)物模型研究、免疫病理機(jī)制探索以及感染性疾病進(jìn)程中巨噬細(xì)胞活化狀態(tài)的評(píng)估，為科研人員在豬源樣本中分析CD163的表達(dá)動(dòng)態(tài)、驗(yàn)證免疫調(diào)控通路及評(píng)估炎癥反應(yīng)程度提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    CD163 ELISA Kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA Kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA Kit; sCD163)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    93.75 ng/mL-6000 ng/mL
  • 靈敏度：
    23.4 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 82-90
    1:2 Average % 94
    Range % 91-97
    1:4 Average % 103
    Range % 100-106
    1:8 Average % 99
    Range % 95-103
  • 回收率：
    The recovery of pig CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 92-98
    EDTA plasma (n=4) 89 86-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    6000 2.111 2.228 2.170 2.003
    3000 1.365 1.455 1.410 1.243
    1500 0.845 0.868 0.857 0.690
    750 0.555 0.574 0.565 0.398
    375 0.369 0.349 0.359 0.192
    187.5 0.282 0.263 0.273 0.106
    93.75 0.235 0.230 0.233 0.066
    0 0.170 0.163 0.167 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role.
  • 基因功能參考文獻(xiàn)：
    1. Study investigated the association of three CD163 polymorphisms with host immune responses following PRRSV challenge and also analyzed the combined effects of the CD163 SNPs. The most significant associations were found with the c.2509G>C, c.2638A>G and c.3534C>T polymorphisms. PMID: 29285769
    2. deletion of Exon 7 of the CD163 gene, encoding SRCR5 had no adverse effects in pigs and these animals showed complete resistance to viral infection by the positive-strand RNA PRRS virus PMID: 28231264
    3. CD163 is not necessary for infection with African swine fever virus. PMID: 27898335
    4. CD163 represents an additional candidate gene for resistance against porcine reproductive and respiratory syndrome. PMID: 26711047
    5. Overexpression of ADAM17 induced downregulation of CD163 expression and a reduction in reproductive and respiratory syndrome virus infection. PMID: 24965453
    6. Fusion protein of sialoadhesin and domains 5-9 of CD163 receptors blocked the respiratory syndrome virus infection. PMID: 24246307
    7. GP4 is involved in interaction with cellular receptor CD163. PMID: 22537809
    8. ). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs. PMID: 21786159
    9. Porcine reproductive and respiratory syndrome virus GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. PMID: 22222209
    10. Our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during porcine reproductive and respiratory syndrome virus replication. PMID: 20496088
    11. The newly established porcine alveolar macrophage cell lines were demonstrated to express robust levels of CD163 and to be fully permissive for both type 1 and 2 porcine reproductive and respiratory syndrome virus strains. PMID: 19900480
    12. The carboxy-terminal 223 residues of the CD163 molecule are not required for interactions with either the GP2a or the GP4 protein, although these residues are required for conferring susceptibility to PRRSV infection. PMID: 19939927
    13. CD163 is shown to be involved in PRRSV (porcine reproductive and respiratory syndrome virus) entry, probably during uncoating, and to interact with sialoadhesin in the process. PMID: 19008379
    14. The expression level of CD163 correlates with the level of PRRSV infection. PMID: 19111584
    15. Clear changes in the quantity of sialoadhesin(+) and CD163(+) macrophages in the placentas and organs of embryos/fetuses during gestation. PMID: 19410291

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  • 亞細(xì)胞定位：
    [Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Expressed in monocytes and macrophages. Detected only in one population of monocytes (CD163+) which is in advanced maturation stage.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ssc:397031

    UniGene: Ssc.100404