Pig Stromal cell derived factor 1a,SDF-1a ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬基質(zhì)細胞衍生因子1a(SDF-1a/CXCL12)酶聯(lián)免疫試劑盒（CSB-E12055p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細胞裂解物樣本中的SDF1a/CXCL12含量。SDF1a/CXCL12 是重要靶點。它作為趨化因子，在細胞遷移、增殖等生理過程起關(guān)鍵作用。在研究機制上，其通過與受體結(jié)合，激活下游信號通路。在腫瘤、炎癥等疾病中也有異常表達和功能，是藥物研發(fā)潛在方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于體外培養(yǎng)細胞模型、動物實驗組織樣本或生物體液中的SDF - 1a動態(tài)監(jiān)測；為探索其在心血管疾病、再生醫(yī)學或免疫微環(huán)境調(diào)控中的功能機制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

SDF1a/CXCL12
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell Lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	86
	Range %	82-90
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	86	82-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.