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  4. Pig apolipoprotein E (Apo-E) ELISA Kit

Pig apolipoprotein E (Apo-E) ELISA Kit

  • 中文名稱(chēng)：
    豬載脂蛋白E(Apo-E)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17889p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬載脂蛋白E(Apo-E)酶聯(lián)免疫試劑盒（CSB-E17889p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOE含量。APOE 即載脂蛋白 E，在脂質(zhì)代謝、神經(jīng)保護(hù)等方面起關(guān)鍵作用。其基因多態(tài)性與阿爾茨海默病、心血管病等密切相關(guān)。研究機(jī)制包括影響脂質(zhì)轉(zhuǎn)運(yùn)、調(diào)節(jié)免疫反應(yīng)、參與神經(jīng)細(xì)胞間信號(hào)傳導(dǎo)，為相關(guān)疾病的診斷和治療提供了重要靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，本產(chǎn)品適用于科研領(lǐng)域中對(duì)豬脂代謝機(jī)制、營(yíng)養(yǎng)干預(yù)模型或相關(guān)病理生理研究的Apo - E水平分析，為動(dòng)物實(shí)驗(yàn)樣本提供穩(wěn)定、特異的檢測(cè)方案本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 96
    Range % 85-105
    1:200 Average % 90
    Range % 89-98
    1:400 Average % 96
    Range % 82-103
    1:800 Average % 97
    Range % 92-99
  • 回收率：
    The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 92-105
    EDTA plasma (n=4) 95 85-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    2000 0.131 0.134 0.133 　
    1000 0.223 0.226 0.225 　
    500 0.335 0.341 0.338 　
    250 0.562 0.553 0.558 　
    125 0.862 0.860 0.861 　
    62.5 1.192 1.214 1.203 　
    31.25 1.589 1.607 1.598 　
    0 2.417 2.395 2.406 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
  • 基因功能參考文獻(xiàn)：
    1. These data suggest that the -155T>A mutation in the promoter region of the porcine APOE gene is an important functional variant PMID: 21549015
  • 亞細(xì)胞定位：
    Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 數(shù)據(jù)庫(kù)鏈接：