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  4. Pig glucagon like peptide 1(GLP1) ELISA Kit

Pig glucagon like peptide 1(GLP1) ELISA Kit

  • 中文名稱：
    豬胰高血糖素樣肽1(GLP1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ027281PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰高血糖素樣肽1(GLP1)酶聯(lián)免疫試劑盒（CSB-EQ027281PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GLP1含量。GLP1即胰高血糖素樣肽-1，是腸道L細(xì)胞分泌的一種肽類激素。它能促進(jìn)胰島素分泌、抑制胰高血糖素分泌，延緩胃排空，從而降低血糖。目前基于GLP1的藥物在糖尿病、肥胖癥等疾病治療領(lǐng)域研究熱度高，為相關(guān)治療帶來新方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)科研領(lǐng)域中對豬模型GLP1分泌調(diào)控機(jī)制的研究、代謝性疾病動物模型評估、以及相關(guān)生物活性分子或藥物干預(yù)效果的體外檢測，為探索GLP1在糖代謝、能量平衡及腸道 - 胰腺軸功能中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    GLP1
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-93  
    1:2 Average % 95  
    Range % 90-105  
    1:4 Average % 98  
    Range % 92-110  
    1:8 Average % 93  
    Range % 85-98  
  • 回收率：
    The recovery of pig GLP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-104  
    EDTA plasma (n=4) 94 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.028 2.014 2.021 1.803  
    500 1.562 1.540 1.551 1.333  
    250 0.949 0.932 0.941 0.723  
    125 0.565 0.568 0.567 0.349  
    62.5 0.351 0.347 0.349 0.131  
    31.2 0.301 0.322 0.312 0.094  
    15.6 0.284 0.286 0.285 0.067  
    0 0.214 0.222 0.218 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

I want to measure total GLP-1 in plasma samples, but I do not understand how it would be biologically relevant to measure 1-37 (with no cross reactivity to other fragments) since GLP-1 is released from the L-cells to the blood stream as either 7-37 or 7-36 and then rapidly degraded to 9-37 and 9-36.
As far as I understand 1-37 is not present in plasma. Could you please explain?

A:
Thanks for your mail!
For the in-body enviroment, the half life of GLP-1 is around 30 seconds. Then GLP-1 cannot be detected in this enviroment.
When the sample prepared and detached from the original in-body enviroment, it can be detected in this condition.
As for the different fragments, it is validated from our side that there is no across activity with another peptide fragments.
Q:

I was wondering if you knew whether the "Pig glucagon like peptide 1(GLP1) ELISA Kit" is meant for pig serum/plasma, or human serum/plasma?

A:
Thanks for your inquir! Pig glucagon like peptide 1(GLP1) ELISA KitCSB-EQ027281PI
This kit is specific to pig. It is suitable to test pig serum/plasma.