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  4. Rat glucagon-like peptide-1,GLP-1 ELISA Kit

Rat glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名稱：
    大鼠胰高血糖素樣肽1(Glp-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08117r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰高血糖素樣肽1(Glp-1)酶聯(lián)免疫試劑盒（CSB-E08117r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Glp1含量。Glp1即胰高血糖素樣肽-1，是腸道L細(xì)胞分泌的一種肽類激素。其背景在于可延緩胃排空、抑制食欲等。研究機制上，它能與相應(yīng)受體結(jié)合，刺激胰島素分泌，降低血糖，同時還參與能量代謝調(diào)控，目前是糖尿病、肥胖癥等疾病藥物研發(fā)熱門靶點。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，該產(chǎn)品適用于糖尿病機制研究、代謝性疾病模型評估、腸道內(nèi)分泌功能分析及藥物開發(fā)中Glp - 1相關(guān)調(diào)控效應(yīng)的體外驗證，為探索胃腸激素信號通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    Glp1
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.625 pg/mL-40 pg/mL
  • 靈敏度：
    0.156 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %84-95
    1:200Average %95
    Range %91-100
    1:400Average %84
    Range %81-89
    1:800Average %92
    Range %86-98
  • 回收率：
    The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)10395-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.607 2.744 2.676 2.531
    202.303 2.451 2.377 2.232
    101.817 1.922 1.870 1.725
    51.133 1.191 1.162 1.017
    2.50.621 0.632 0.627 0.482
    1.250.344 0.355 0.350 0.205
    0.6250.234 0.248 0.241 0.096
    00.140 0.150 0.145 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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