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Pig oxytocin,OT ELISA Kit

  • 中文名稱:
    豬催產(chǎn)素(OT)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E12062p
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬催產(chǎn)素(OT)酶聯(lián)免疫試劑盒(CSB-E12062p)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的OT含量。試劑盒檢測(cè)范圍為2.5 pg/mL-100 pg/mL,科研人員可通過探究母豬分娩機(jī)制、泌乳調(diào)控規(guī)律,或分析OT在社交行為及環(huán)境適應(yīng)中的分子調(diào)控網(wǎng)絡(luò),尤其適用于比較醫(yī)學(xué)模型構(gòu)建及畜牧養(yǎng)殖相關(guān)激素代謝研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    MGC126890, MGC126892, OT, OT-NPI, neurophysin I|oxytocin, prepro- (neurophysin I)|oxytocin-neurophysin I|oxytocin-neurophysin I, preproprotein
  • 縮寫:
    OT
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    2.5 pg/mL-100 pg/mL
  • 靈敏度:
    2.5 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig oxytocin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 91-105  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 92  
    Range % 87-99  
    1:8 Average % 95  
    Range % 90-101  
  • 回收率:
    The recovery of pig oxytocin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-96  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 2.451 2.428 2.440    
    2.5 1.052 1.029 1.041    
    7.5 0.551 0.536 0.544    
    23 0.381 0.392 0.387    
    50 0.275 0.262 0.269    
    100 0.144 0.142 0.143    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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