Rat oxytocin,OT ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠催產(chǎn)素(OT)酶聯(lián)免疫試劑盒（CSB-E14197r）為競爭法ELISA試劑盒，定量檢測血清、血漿.樣本中的OT含量。大鼠催產(chǎn)素（OT）是一種由九個氨基酸組成的神經(jīng)肽，主要在下丘腦室旁核和視上核合成，通過激活G蛋白偶聯(lián)受體（GPCR）發(fā)揮功能，調(diào)節(jié)分娩、哺乳、社交行為等。其釋放的時空動態(tài)及分子機制尚不明晰，近年來，北京大學(xué)生命科學(xué)學(xué)院李毓龍實驗室開發(fā)了新型催產(chǎn)素?zé)晒馓结楪RABOT1.0，深入探究了催產(chǎn)素釋放的分子機制。試劑盒檢測范圍為7.5 pg/mL-600 pg/mL，適用于神經(jīng)內(nèi)分泌研究、行為學(xué)實驗及壓力反應(yīng)機制等科研領(lǐng)域。科研人員可通過該產(chǎn)品深入探究OT在焦慮調(diào)控、分娩啟動或社會互動中的分子機制，為神經(jīng)生物學(xué)和內(nèi)分泌學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

OT
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma.
檢測范圍：

7.5 pg/mL-600 pg/mL
靈敏度：

9.375 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-102
1:2	Average %	93
	Range %	90-96
1:4	Average %	98
	Range %	95-102
1:8	Average %	101
	Range %	98-104

回收率：

The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	89-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.