Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒（CSB-E06856Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Col 含量。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研場景中軟骨相關(guān)疾病模型研究，如關(guān)節(jié)炎病理機(jī)制探索、軟骨損傷修復(fù)評估或藥物干預(yù)效果驗(yàn)證等領(lǐng)域，為組織工程、生物材料開發(fā)及退行性疾病研究提供可靠的檢測工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
縮寫：

Col
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-102
1:2	Average %	107
	Range %	97-111
1:4	Average %	88
	Range %	82-92
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-94
EDTA plasma (n=4)	102	92-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.