Rabbit Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子Ⅳ型膠原(Col Ⅳ)酶聯(lián)免疫試劑盒（CSB-E14232Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col 含量。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，可應(yīng)用于基礎(chǔ)研究領(lǐng)域，如探究腎臟、肝臟等器官纖維化模型中的基底膜代謝異常，分析腫瘤微環(huán)境中基質(zhì)重構(gòu)機(jī)制，或評估血管病變相關(guān)疾病模型中Col IV的動態(tài)變化?？蒲腥藛T可通過血清/血漿樣本實現(xiàn)縱向監(jiān)測，或利用組織勻漿進(jìn)行局部表達(dá)水平解析，為組織工程、病理機(jī)制及藥物干預(yù)研究提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
縮寫：

Col
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	93
	Range %	89-97
1:4	Average %	98
	Range %	95-104
1:8	Average %	86
	Range %	80-94

回收率：

The recovery of rabbit COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	93	87-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.