Rabbit Complement 3,C3 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔補體蛋白3(C3)酶聯(lián)免疫試劑盒（CSB-E08668Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的C3含量。C3作為靶點在生物醫(yī)學(xué)領(lǐng)域受關(guān)注。它是補體系統(tǒng)關(guān)鍵成分。研究表明，C3參與免疫反應(yīng)與炎癥進(jìn)程，異常激活影響多種疾病發(fā)展。針對其的研究機(jī)制聚焦在阻斷異常激活途徑，期望開發(fā)出能治療相關(guān)疾病的療法。試劑盒檢測范圍為27.3 ng/mL-7000 ng/mL，適用于兔類動物模型中的免疫調(diào)控研究，如感染性疾病機(jī)制探索、自身免疫病理分析或炎癥相關(guān)藥物開發(fā)中的生物標(biāo)志物檢測，為科研人員提供標(biāo)準(zhǔn)化的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
縮寫：

C3
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

27.3 ng/mL-7000 ng/mL
靈敏度：

27.3 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	91-110
1:2000	Average %	89
	Range %	82-99
1:4000	Average %	95
	Range %	85-99
1:8000	Average %	91
	Range %	87-98

回收率：

The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-97
EDTA plasma (n=4)	97	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.