Rabbit Testosterone,T ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子睪酮(T)酶聯(lián)免疫試劑盒（CSB-E06927Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的T含量。試劑盒檢測范圍為0.625 ng/mL-10 ng/mL，適用于實驗室環(huán)境下兔睪酮水平的動態(tài)監(jiān)測，如性腺功能研究、激素代謝調(diào)控實驗、藥物干預(yù)效果評價等基礎(chǔ)科研領(lǐng)域；對動物內(nèi)分泌機制研究、生殖健康評估及激素相關(guān)疾病模型構(gòu)建具有重要價值本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

T
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-10 ng/mL
靈敏度：

0.625 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	92
	Range %	88-99

回收率：

The recovery of rabbit testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-92
EDTA plasma (n=4)	93	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.