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  4. Rat Testosterone,T ELISA Kit

Rat Testosterone,T ELISA Kit

  • 中文名稱：
    大鼠睪酮(T)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E05100r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠睪酮(T)酶聯(lián)免疫試劑盒（CSB-E05100r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的T含量。大鼠睪酮（T）是男性激素，對生殖功能和男性特征發(fā)育至關(guān)重要。其合成主要在睪丸間質(zhì)細胞（Leydig細胞）中進行，受促性腺激素（LH）調(diào)控。睪酮水平異常與多種生殖和生理問題相關(guān)，如少弱精子癥、性功能障礙等。研究睪酮的作用機制有助于開發(fā)治療相關(guān)疾病的藥物和方法。試劑盒檢測范圍為0.13 ng/mL-25.6 ng/mL，靈敏度為0.06 ng/mL。動物實驗研究中的激素水平監(jiān)測，如生殖生物學機制探索、藥物或環(huán)境因素對內(nèi)分泌系統(tǒng)的影響評估、以及疾病模型（如代謝綜合征或性腺功能障礙）的睪酮動態(tài)變化分析等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    T
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.13 ng/mL-25.6 ng/mL
  • 靈敏度：
    0.06 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-104  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 89  
    Range % 83-93  
  • 回收率：
    The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-95  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    25.6 0.238 0.236 0.237    
    6.4 0.435 0.431 0.433    
    2 0.611 0.615 0.613    
    0.5 0.984 0.982 0.983    
    0.13 1.403 1.398 1.401    
    0 2.001 2.003 2.002    
  • 本試劑盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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產(chǎn)品評價

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