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  4. Rabbit von Willebrand Factor,VWF ELISA Kit

Rabbit von Willebrand Factor,VWF ELISA Kit

  • 中文名稱(chēng)：
    兔血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08525Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒（CSB-E08525Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的VWF含量。VWF（血管性血友病因子）在止血和血栓形成中起關(guān)鍵作用。其背景上，VWF缺陷或異常會(huì)導(dǎo)致血管性血友病。研究機(jī)制方面，它能與血小板和內(nèi)皮下膠原結(jié)合，介導(dǎo)血小板黏附、聚集，還參與凝血因子Ⅷ的運(yùn)輸和保護(hù)，維持其活性。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品廣泛應(yīng)用于心血管疾病動(dòng)物模型中的凝血機(jī)制研究、抗血栓藥物體外作用評(píng)估以及血小板功能相關(guān)的基礎(chǔ)科研實(shí)驗(yàn)，可為血管內(nèi)皮損傷、血栓形成等病理過(guò)程的分子機(jī)制探索提供數(shù)據(jù)支持，滿(mǎn)足實(shí)驗(yàn)室對(duì)VWF蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %95
    Range %85-100
    1:4Average %95
    Range %87-106
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rabbit VWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-103
    EDTA plasma (n=4)9486-100
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.780 2.563 2.672 2.576
    102.266 2.128 2.197 2.101
    51.549 1.475 1.512 1.416
    2.50.907 0.899 0.903 0.807
    1.250.465 0.434 0.450 0.354
    0.6250.245 0.239 0.242 0.146
    0.3120.153 0.148 0.151 0.055
    00.099 0.093 0.096 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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