Rat C-telopeptide of type Ⅱcollagen,CTX-Ⅱ ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯免疫試劑盒（CSB-E12963r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，本試劑盒適用于體外定量分析，可支持科研領域對軟骨代謝機制、藥物干預效果及疾病動物模型的評估；在骨關節(jié)退行性病變、類風濕性關節(jié)炎等軟骨損傷相關疾病的研究中具有重要價值；為軟骨健康相關的分子機制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C-telopeptide of type 2 collagen,CTX-2,_x000D_ C-telopeptide of type II collagen,CTX-II
縮寫：

CTX-Ⅱ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-94
1:2	Average %	101
	Range %	97-104
1:4	Average %	91
	Range %	87-94
1:8	Average %	98
	Range %	95-103

回收率：

The recovery of rat CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	95	91-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.