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Rat Factor-related Apoptosis,FAS ELISA Kit

  • 中文名稱:
    大鼠凋亡相關(guān)因子(FAS/CD95)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07324r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠凋亡相關(guān)因子(FAS/CD95)酶聯(lián)免疫試劑盒(CSB-E07324r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的FAS含量。FAS(Fas/FasL系統(tǒng))是細(xì)胞凋亡信號(hào)轉(zhuǎn)導(dǎo)途徑的主要系統(tǒng),由Fas(APO-1/CD95)和其配體FasL組成。Fas/FasL系統(tǒng)通過死亡受體與配體結(jié)合,激活下游的Caspase-8和Caspase-3,進(jìn)而引發(fā)細(xì)胞凋亡。該系統(tǒng)在免疫監(jiān)視、腫瘤發(fā)生和免疫逃逸中發(fā)揮重要作用。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL,F(xiàn)AS/CD95作為細(xì)胞表面凋亡信號(hào)受體,廣泛參與細(xì)胞程序性死亡、免疫穩(wěn)態(tài)調(diào)控及腫瘤發(fā)生等生物學(xué)過程,本試劑盒適用于神經(jīng)退行性疾病、自身免疫疾病及癌癥機(jī)制等研究領(lǐng)域,幫助科研人員分析細(xì)胞凋亡相關(guān)通路中FAS/CD95的表達(dá)水平。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Fas ELISA Kit; Apt1 ELISA Kit; Tnfrsf6 ELISA Kit; Tumor necrosis factor receptor superfamily member 6 ELISA Kit; Apo-1 antigen ELISA Kit; Apoptosis-mediating surface antigen FAS ELISA Kit; FASLG receptor ELISA Kit; CD antigen CD95 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 ng/mL-50 ng/mL
  • 靈敏度:
    0.195 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %86-105
    1:2Average %96
    Range %91-100
    1:4Average %96
    Range %87-105
    1:8Average %96
    Range %90-102
  • 回收率:
    The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-105
    EDTA plasma (n=4)9593-102
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.504 2.532 2.518 2.402
    252.312 2.345 2.329 2.213
    12.51.884 1.879 1.882 1.766
    6.251.111 1.133 1.122 1.006
    3.120.573 0.586 0.580 0.464
    1.560.363 0.374 0.369 0.253
    0.780.241 0.255 0.248 0.132
    00.115 0.117 0.116  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
  • 基因功能參考文獻(xiàn):
    1. Report dysregulation of the Fas/FasL system occurs in an experimental animal model of HELLP syndrome. PMID: 28501275
    2. Vitamin D could alleviate pathological changes, reduce Fas/FasL expression, and attenuate myocardial cell apoptosis in diabetic cardiomyopathy rats, which might be used as a potential effective therapy for the disease. PMID: 28595623
    3. Study found that propofol anesthesia activates Fas/FasL-mediated extrinsic and Bcl-2-dependent intrinsic apoptotic pathways, resulting in caspase-8 and caspase-9 activations, respectively, and established upregulation of caspase-1 and IL-1beta cytokine transcriptions as well as subsequent activation of microglia. Behavioral data revealed that hyperactivity persists through adolescence and young adulthood. PMID: 27189477
    4. NaIO3 activates Fas-mediated cell death, both in the RPE and photoreceptor, and that a small peptide antagonist of the Fas receptor, Met12, significantly reduces the extent of this cell death. PMID: 28346613
    5. Western blotting demonstrated that expression of CD95, FasL and FADD was very weak during embryonic development, but rapidly increased at P14. PMID: 27561622
    6. miR-133b-5p might play a particularly important role in the cardioprotective effect of morphine preconditioning by regulating the target gene Fas PMID: 27919821
    7. Organic pollutants in drinking water impair liver function, with the involvement of Fas and mitochondria-mediated apoptosis in rats. PMID: 26316710
    8. Mip1 over-expression inhibited H2O2-induced apoptosis and upregulation of Fas induced by H2O2 in H9c2 myogenic cells. PMID: 25310648
    9. Chronic methamphetamine exposure will activate cardiac Fas-dependent and mitochondria-dependent apoptotic pathways. PMID: 24307234
    10. Fas protein was up-regulated in rat mesangial cells, and its neutralizing antibody ZB4 partly inhibited M2 macrophage-induced apoptosis PMID: 23973665
    11. The expression of Fas in spermatogenic cells and the sperm aberration of each fluorotic group were higher than control group. PMID: 20568470
    12. Autophagy activation occurs in photoreceptors after retina-retinal pigment epithelium separation. This appears to be, at least in part, dependent on Fas receptor activation and plays role in regulating level of photoreceptor apoptosis. PMID: 21421874
    13. The treatment with cefoperazone sodium and levofloxacin lactate could inhibit the expression of Fas and Bax and enhance the expression of Bcl-2 in rats infected with Vibrio vulnificus. PMID: 19399403
    14. Inhibition of Fas can diminish LPS-induced apoptosis and inflammatory cytokine production in type II alveolar epithelial cell. PMID: 19823951
    15. increased expression on conjunctival epithelial cells of patients with cystic fibrosis PMID: 19642903
    16. Aluminium could influence the activities of learning and memory and could reduce the expression of Bcl-2 and promote the expression of Fas. PMID: 19267062
    17. Results indicate that cytokine-treated beta-cells may possess two pools of Fas protein, one of which is inducible by cytokines and accounts for surface Fas expression, and one which is constitutively expressed in cytoplasmic compartments. PMID: 12683940
    18. critical tyrosine residues of CD95 are targets of the anti-apoptotic action of nitric oxide PMID: 14679192
    19. CD95 regulation involves complex phosphorylations with CD95-tyrosine phosphorylation favoring CD95 membrane trafficking and DISC formation, whereas CD95 Ser/Thr phosphorylation inhibits these processes. PMID: 14699504
    20. epidermal growth factor receptor and CD95 activation are triggered by Src family kinase Yes PMID: 15039424
    21. FAS-mediated apoptosis pathway becomes activated and transcriptionally upregulated after retinal detachment. The peak of FAS activation precedes that of the intrinsic pathway, and inhibition of FAS activation can decrease caspase-9 activity. PMID: 15557468
    22. Adult spinal motor neuron apoptosis is mediated by upstream NO and ONOO- genotoxicity and downstream p53 and Fas activation and is shifted to necrosis by mutant SOD1. PMID: 16000635
    23. In rat islets, constitutive expression of Fas ligand and glucose-induced Fas receptor expression were observed only in 7- to 8-month-old but not in 2- to 3-month-old islets PMID: 16936193
    24. Activation of glial cells and upregulation of Fas suggest that glial cells may contribute to Fas-mediated cell death in the neurodegeneration process of chronic ocular hypertensive retinal insult. PMID: 17045251
    25. Data strongly suggests that transcriptional up regulation of Fas could predispose a group of spermatocytes to Fas ligand triggering apoptosis by the extrinsic and intrinsic pathway. PMID: 17195944
    26. These results suggest that Hsp70 is capable of protecting the cardiomyocyte from stress-induced injury by inhibiting Fas-mediated apoptosis. PMID: 17441510
    27. Apoptosis in our model occurs via both extrinsic and intrinsic pathways, and activation of Fas may play a role. PMID: 18289516
    28. CDK inhibitors suppress MDM2 levels and enhance p53 expression that facilitates bile acid-induced, ceramide-dependent CD95 activation to induce both apoptosis and autophagy in primary hepatocytes PMID: 18614532
    29. Using single-particle EM, we have identified a near continuum of conformations consistent with a remarkable flexibility of FAS. PMID: 19151726

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  • 亞細(xì)胞定位:
    Cell membrane; Single-pass type I membrane protein. Membrane raft.
  • 數(shù)據(jù)庫鏈接: