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  4. Rat Folic acid,FA ELISA Kit

Rat Folic acid,FA ELISA Kit

  • 中文名稱：
    大鼠葉酸(FA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08757r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠葉酸(FA)酶聯(lián)免疫試劑盒（CSB-E08757r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FA含量。葉酸是一種水溶性B族維生素，對DNA合成、修復和甲基化至關重要。在大鼠研究中，葉酸缺乏會影響生長發(fā)育，可能通過調(diào)節(jié)胰島素樣生長因子（IGFs）系統(tǒng)的甲基化水平，進而影響胚胎發(fā)育和細胞增殖。葉酸還與DNA甲基化、細胞代謝和神經(jīng)管缺陷等過程密切相關。試劑盒檢測范圍為19.5 pg/mL-5000 pg/mL，靈敏度為19.5 pg/mL。本試劑盒適用于科研領域中對大鼠葉酸代謝相關研究，包括膳食營養(yǎng)評估、葉酸缺乏模型構建、藥物干預效果分析等基礎實驗，為探索葉酸在生長發(fā)育、造血功能及神經(jīng)系統(tǒng)中的作用機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    FA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    19.5 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 86-104  
    1:2 Average % 92  
    Range % 83-99  
    1:4 Average % 96  
    Range % 87-98  
    1:8 Average % 94  
    Range % 80-98  
  • 回收率：
    The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 86-102  
    EDTA plasma (n=4) 94 88-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.324 0.326 0.325    
    1250 0.514 0.528 0.521    
    312 0.817 0.814 0.816    
    78 1.281 1.293 1.287    
    19.5 1.674 1.635 1.655    
    0 1.948 1.966 1.957    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關問答

■ 常見問題解答
Q:

Regarding CSB-E08757r-1plate-we would like to know the origin of the standard Folic Acid? Is it derived from rat or baculovirus etc?

A:
Thanks for your inquiry.
The standard is purified small molecule. Sorry we can not disclose the specific origin. Hope you could understand.
Q:

Sorry to bothering you, and I have questions about your product CSB-E08757r.
1) Could you let us know the chemical name and concentration of acid in Stop Solution?
2) Could you let us know whether any preservatives are contained in each component?”.

A:
Thanks for your inquiry.
CSB-E08757r
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).

靶點詳情

  • 最新研究進展：
    葉酸（Folic acid）是一種維生素B族成員，參與人體DNA、RNA的合成和修復，對于細胞生長和分裂非常重要。最新的研究表明，葉酸在人體內(nèi)具有多種生理功能，包括對于心血管健康和神經(jīng)系統(tǒng)健康的影響，以及對于出生缺陷和某些類型的癌癥的預防作用。